Transport kinetics of uncoupling proteins: analysis of UCP1 reconstituted in planar lipid bilayers.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F03%3A20030099" target="_blank" >RIV/67985823:_____/03:20030099 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Transport kinetics of uncoupling proteins: analysis of UCP1 reconstituted in planar lipid bilayers.

Popis výsledku v původním jazyce

According to alternative hypotheses, mitochondrial uncoupling protein 1 (UCP1) is either a proton channel (buffering model) or a fatty acid anion carrier (fatty acid cycling). Transport across the proton channel along a chain of hydrogen bonds (Grotthusmechanism) may include fatty acid carboxyl groups or occur in the absence of fatty acids. In the present work we demonstrate that planar bilayers reconstituted with UCP1 exhibit an increase in membrane conductivity exclusively in the presence of fatty acids (FA). Hence we can exclude the hypothesis, considering a pre-existing H+ channel in UCP1 which does not require FA for function. The augmented conductivity is nearly completely blocked by ATP. Direct application of transmembrane voltage and precise current measurements allowed determination of ATP-sensitive conductances at 0 and 150 mV as 11.5 pS and 54.3 pS, respectively, by reconstituting nearly 3*105 copies of UCP1. The proton conductivity measurements carried out in presence of a

Název v anglickém jazyce

Transport kinetics of uncoupling proteins: analysis of UCP1 reconstituted in planar lipid bilayers.

Popis výsledku anglicky

According to alternative hypotheses, mitochondrial uncoupling protein 1 (UCP1) is either a proton channel (buffering model) or a fatty acid anion carrier (fatty acid cycling). Transport across the proton channel along a chain of hydrogen bonds (Grotthusmechanism) may include fatty acid carboxyl groups or occur in the absence of fatty acids. In the present work we demonstrate that planar bilayers reconstituted with UCP1 exhibit an increase in membrane conductivity exclusively in the presence of fatty acids (FA). Hence we can exclude the hypothesis, considering a pre-existing H+ channel in UCP1 which does not require FA for function. The augmented conductivity is nearly completely blocked by ATP. Direct application of transmembrane voltage and precise current measurements allowed determination of ATP-sensitive conductances at 0 and 150 mV as 11.5 pS and 54.3 pS, respectively, by reconstituting nearly 3*105 copies of UCP1. The proton conductivity measurements carried out in presence of a

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/IAA5011106" target="_blank" >IAA5011106: Studium nových mitochondriálních odpřahujících proteinů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biological Chemistry

ISSN

0021-9258

e-ISSN

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Svazek periodika

278

Číslo periodika v rámci svazku

35

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

32497-32500

Kód UT WoS článku

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EID výsledku v databázi Scopus

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