Role negativně nabitých aminokyselin v F-kličce beta4 podjednotky při aktivaci a desenzitizaci alfa3beta4 potkaního neuronálního nikotinického receptoru

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F08%3A00306860" target="_blank" >RIV/67985823:_____/08:00306860 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Role of negatively charged amino acids in beta4 F-loop in activation and desensitization of alfa3beta4 rat neuronal nicotinic receptors

Popis výsledku v původním jazyce

Mutation of aspartates in positions 191 and 192 of the F-loop of beta4 subunit to their uncharged analogs asparagines led to a receptor with lowered rate of desensitization and accelerated resensitization of responses to agonists as measured by whole-cell patch-clamp technique. Sensitivity of the mutated receptors to ligands is shifted towards higher concentrations, which is substantially more pronounced in case of agonists than in competitive antagonist. Some agonists did not activate the mutated variant of the receptor at all but their ability to bind was preserved and such ligands acted as competitive antagonists of the mutated receptors. Concluding: aspartates in the loop F do not directly take part in ligand binding but they are essential for proper receptor gating

Název v anglickém jazyce

Role of negatively charged amino acids in beta4 F-loop in activation and desensitization of alfa3beta4 rat neuronal nicotinic receptors

Popis výsledku anglicky

Mutation of aspartates in positions 191 and 192 of the F-loop of beta4 subunit to their uncharged analogs asparagines led to a receptor with lowered rate of desensitization and accelerated resensitization of responses to agonists as measured by whole-cell patch-clamp technique. Sensitivity of the mutated receptors to ligands is shifted towards higher concentrations, which is substantially more pronounced in case of agonists than in competitive antagonist. Some agonists did not activate the mutated variant of the receptor at all but their ability to bind was preserved and such ligands acted as competitive antagonists of the mutated receptors. Concluding: aspartates in the loop F do not directly take part in ligand binding but they are essential for proper receptor gating

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica Et Biophysica Acta-Biomembranes

ISSN

0005-2736

e-ISSN

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Svazek periodika

1778

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

864-871

Kód UT WoS článku

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EID výsledku v databázi Scopus

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