Plasma Membrane Density of GABA(B)-R1a, GABA(B)-R1b, GABA-R2 and Trimeric G-proteins in the Course of Postnatal Development of Rat Brain Cortex

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Plasma Membrane Density of GABA(B)-R1a, GABA(B)-R1b, GABA-R2 and Trimeric G-proteins in the Course of Postnatal Development of Rat Brain Cortex

Popis výsledku v původním jazyce

GABAB-R1a, GABAB-R1b, GABAB-R2, Gi1/Gi2alpha, Gi3alpha, Goalpha, Gzalpha and Gbeta subunit protein content was determined by quantitative immunoblotting in PM isolated from brain cortex of rats of different ages: between postnatal day1 (PD1) and 90 (PD90). PM density of GABAB-R1alpha, GABAB-R2, Gi1/Gi2alpha, Gi3alpha, Goalpha, Gzalpha and Gbeta was high at birth. The intensity of GABAB-R1a and GABAB-R2 subunits signal decreased between the birth and PD15: to 55 % (R1a, **) and 51 % (R2, **), respectively. Contrarily, PM level of the cognate G-proteins Gi1/Gi2alpha, Gi3alpha, Goalpha, Gzalpha and Gbeta was unchanged in the course of the whole postnatal period of brain cortex development. Maturation of GABAB-R cascade was substantially different from ontogenetic profile of Na, K-ATPase, which was low at birth and increased between the birth and PD25. In adult rats, membrane content of Na, K-ATPase was 3-times higher than around the birth

Název v anglickém jazyce

Plasma Membrane Density of GABA(B)-R1a, GABA(B)-R1b, GABA-R2 and Trimeric G-proteins in the Course of Postnatal Development of Rat Brain Cortex

Popis výsledku anglicky

GABAB-R1a, GABAB-R1b, GABAB-R2, Gi1/Gi2alpha, Gi3alpha, Goalpha, Gzalpha and Gbeta subunit protein content was determined by quantitative immunoblotting in PM isolated from brain cortex of rats of different ages: between postnatal day1 (PD1) and 90 (PD90). PM density of GABAB-R1alpha, GABAB-R2, Gi1/Gi2alpha, Gi3alpha, Goalpha, Gzalpha and Gbeta was high at birth. The intensity of GABAB-R1a and GABAB-R2 subunits signal decreased between the birth and PD15: to 55 % (R1a, **) and 51 % (R2, **), respectively. Contrarily, PM level of the cognate G-proteins Gi1/Gi2alpha, Gi3alpha, Goalpha, Gzalpha and Gbeta was unchanged in the course of the whole postnatal period of brain cortex development. Maturation of GABAB-R cascade was substantially different from ontogenetic profile of Na, K-ATPase, which was low at birth and increased between the birth and PD25. In adult rats, membrane content of Na, K-ATPase was 3-times higher than around the birth

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

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Svazek periodika

62

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

13

Strana od-do

547-559

Kód UT WoS článku

000327444700009

EID výsledku v databázi Scopus

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