Coupled aggregation of mitochondrial single-strand DNA-binding protein tagged with Eos fluorescent protein visualizes synchronized activity of mitochondrial nucleoids
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F15%3A00449774" target="_blank" >RIV/67985823:_____/15:00449774 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.3892/mmr.2015.4085" target="_blank" >http://dx.doi.org/10.3892/mmr.2015.4085</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3892/mmr.2015.4085" target="_blank" >10.3892/mmr.2015.4085</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Coupled aggregation of mitochondrial single-strand DNA-binding protein tagged with Eos fluorescent protein visualizes synchronized activity of mitochondrial nucleoids
Popis výsledku v původním jazyce
A small limited number of large aggregates of mtSSB EosFP tagged protein started to form in the cells, which exhibited a great co localization with the noted coupled positions. Antibody staining and 3D immunocytochemistry confirmed that nucleoid components such as TFAM and DNA were co localized with these aggregates. Furthermore, the observed reduction of the mtDNA copy number in mtSSB EosFP transfected cells suggested a possible impairment of nucleoid function. In conclusion, the present study demonstrated that coupled nucleoids are synchronized by mtSSB EosFP overexpression and visualized through their equal binding capacity to mtSSB EosFP tagged protein. This observation suggested parallel replication and transcription activity of nucleoid couples native from a parental one. Preserved coupling in late stages of artificial EosFP mediated aggregation of tagged proteins suggested a rational manner of mitochondrial branching that may be cell-type specifically dependent on hierarchical n
Název v anglickém jazyce
Coupled aggregation of mitochondrial single-strand DNA-binding protein tagged with Eos fluorescent protein visualizes synchronized activity of mitochondrial nucleoids
Popis výsledku anglicky
A small limited number of large aggregates of mtSSB EosFP tagged protein started to form in the cells, which exhibited a great co localization with the noted coupled positions. Antibody staining and 3D immunocytochemistry confirmed that nucleoid components such as TFAM and DNA were co localized with these aggregates. Furthermore, the observed reduction of the mtDNA copy number in mtSSB EosFP transfected cells suggested a possible impairment of nucleoid function. In conclusion, the present study demonstrated that coupled nucleoids are synchronized by mtSSB EosFP overexpression and visualized through their equal binding capacity to mtSSB EosFP tagged protein. This observation suggested parallel replication and transcription activity of nucleoid couples native from a parental one. Preserved coupling in late stages of artificial EosFP mediated aggregation of tagged proteins suggested a rational manner of mitochondrial branching that may be cell-type specifically dependent on hierarchical n
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EA - Morfologické obory a cytologie
OECD FORD obor
—
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Molecular Medicine Reports
ISSN
1791-2997
e-ISSN
—
Svazek periodika
12
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
GR - Řecká republika
Počet stran výsledku
6
Strana od-do
5185-5190
Kód UT WoS článku
000362999200051
EID výsledku v databázi Scopus
2-s2.0-84940564235