Analysis of Whole-Cell NMDA Receptor Currents

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00459532" target="_blank" >RIV/67985823:_____/16:00459532 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-4939-2812-5_14" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-2812-5_14</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-2812-5_14" target="_blank" >10.1007/978-1-4939-2812-5_14</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of Whole-Cell NMDA Receptor Currents

Popis výsledku v původním jazyce

During receptor channel activation, the receptor goes through a ligand binding step followed by conformation steps leading to the open channel conformation. The probability of finding the receptor in a particular conformation is given by the apparent rate constants. The apparent rate constants describe the rate of transitions between conformational states and vary among different types of iGluR. To determine these parameters reflecting receptor channel function, several approaches have been developed. In this chapter we describe methods for the measurement of basic kinetic parameters of the NMDA receptor determined from non-stationary analysis of whole-cell currents. Particularly, the apparent rate constants of agonist binding/unbinding, desensitization/resensitization, and the probability of channel opening are determined. Besides the kinetic parameters of the receptor, the number of ion channels in the cell membrane and single-channel conductance are estimated from whole-cell recordings.

Název v anglickém jazyce

Analysis of Whole-Cell NMDA Receptor Currents

Popis výsledku anglicky

During receptor channel activation, the receptor goes through a ligand binding step followed by conformation steps leading to the open channel conformation. The probability of finding the receptor in a particular conformation is given by the apparent rate constants. The apparent rate constants describe the rate of transitions between conformational states and vary among different types of iGluR. To determine these parameters reflecting receptor channel function, several approaches have been developed. In this chapter we describe methods for the measurement of basic kinetic parameters of the NMDA receptor determined from non-stationary analysis of whole-cell currents. Particularly, the apparent rate constants of agonist binding/unbinding, desensitization/resensitization, and the probability of channel opening are determined. Besides the kinetic parameters of the receptor, the number of ion channels in the cell membrane and single-channel conductance are estimated from whole-cell recordings.

Druh

C - Kapitola v odborné knize

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Ionotropic Glutamate Receptor Technologies

ISBN

978-1-4939-2811-8

Počet stran výsledku

15

Strana od-do

205-219

Počet stran knihy

304

Název nakladatele

Humana Press Inc.

Místo vydání

Totowa

Kód UT WoS kapitoly

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