Counting NMDA Receptors at the Cell Surface

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00459534" target="_blank" >RIV/67985823:_____/16:00459534 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-4939-2812-5_3" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-2812-5_3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-2812-5_3" target="_blank" >10.1007/978-1-4939-2812-5_3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Counting NMDA Receptors at the Cell Surface

Popis výsledku v původním jazyce

In the mammalian central nervous system, N-methyl-d-aspartate (NMDA) receptors mediate fast excitatory synaptic transmission. These receptors also play a key role in brain development, synaptic plasticity, and memory formation, and their dysregulation can cause neurological and/or psychiatric disorders. A variety of techniques have been applied to examine the molecular mechanisms that regulate the number of NMDA receptors at the cell surface. Here we provide a step-by-step guides for two commonly used techniques: a quantitative assay that measures both surface and total receptor pools, and a biotinylation assay that can rapidly evaluate the surface and total expression levels of receptor subunits. Among other applications, these methods can be used to measure the cell surface expression of recombinant receptors with genetic alterations and the effect of co-expressing additional proteins that may regulate subunit surface trafficking. These techniques can be used either individually or in parallel experiments, thus allowing researchers to independently validate observations regarding the regulation of NMDA receptor expression.

Název v anglickém jazyce

Counting NMDA Receptors at the Cell Surface

Popis výsledku anglicky

In the mammalian central nervous system, N-methyl-d-aspartate (NMDA) receptors mediate fast excitatory synaptic transmission. These receptors also play a key role in brain development, synaptic plasticity, and memory formation, and their dysregulation can cause neurological and/or psychiatric disorders. A variety of techniques have been applied to examine the molecular mechanisms that regulate the number of NMDA receptors at the cell surface. Here we provide a step-by-step guides for two commonly used techniques: a quantitative assay that measures both surface and total receptor pools, and a biotinylation assay that can rapidly evaluate the surface and total expression levels of receptor subunits. Among other applications, these methods can be used to measure the cell surface expression of recombinant receptors with genetic alterations and the effect of co-expressing additional proteins that may regulate subunit surface trafficking. These techniques can be used either individually or in parallel experiments, thus allowing researchers to independently validate observations regarding the regulation of NMDA receptor expression.

Druh

C - Kapitola v odborné knize

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

<a href="/cs/project/GA14-02219S" target="_blank" >GA14-02219S: Regulace transportu a funkce NMDA receptorů N-glykosylací</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Ionotropic Glutamate Receptor Technologies

ISBN

978-1-4939-2811-8

Počet stran výsledku

13

Strana od-do

31-44

Počet stran knihy

304

Název nakladatele

Humana Press Inc.

Místo vydání

Totowa

Kód UT WoS kapitoly

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