Induction of lipogenesis in white fat during cold exposure in mice: link to lean phenotype

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F17%3A00474022" target="_blank" >RIV/67985823:_____/17:00474022 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1038/ijo.2016.228" target="_blank" >http://dx.doi.org/10.1038/ijo.2016.228</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/ijo.2016.228" target="_blank" >10.1038/ijo.2016.228</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Induction of lipogenesis in white fat during cold exposure in mice: link to lean phenotype

Popis výsledku v původním jazyce

Futile substrate cycling based on lipolytic release of fatty acids (FA) from intracellular triacylglycerols (TAG) and their re-esterification (TAG/FA cycling), as well as de novo FA synthesis (de novo lipogenesis (DNL)), represent the core energy-consuming biochemical activities of white adipose tissue (WAT). We aimed to characterize their roles in cold-induced thermogenesis and energy homeostasis.Male obesity-resistant A/J and obesity-prone C57BL/6J mice maintained at 30 degrees C were exposed to 6 degrees C for 2 or 7 days. In epididymal WAT (eWAT), TAG synthesis and DNL were determined using in vivo H-2 incorporation from (H2O)-H-2 into tissue TAG and nuclear magnetic resonance spectroscopy. Quantitative real-time-PCR and/or immunohistochemistry and western blotting were used to determine the expression of selected genes and proteins in WAT and liver.The mass of WAT depots declined during cold exposure (CE). Plasma levels of TAG and non-esterified FA were decreased by day 2 but tended to normalize by day 7 of CE. TAG synthesis (reflecting TAG/FA cycle activity) gradually increased during CE. DNL decreased by day 2 of CE but increased several fold over the control values by day 7. Expression of genes involved in lipolysis, glyceroneogenesis, FA re-esterification, FA oxidation and mitochondrial biogenesis in eWAT was induced during CE. All these changes were more pronounced in obesity-resistant A/J than in B6 mice and occurred in the absence of uncoupling protein 1 in eWAT. Leptin and fibroblast growth factor 21 plasma levels were differentially affected by CE in the two mouse strains. Our results indicate integrated involvement of (i) TAG/FA cycling and DNL in WAT, and (ii) hepatic very-low-density lipoprotein-TAG synthesis in the control of blood lipid levels and provision of FA fuels for thermogenesis in cold. They suggest that lipogenesis in WAT contributes to a lean phenotype.

Název v anglickém jazyce

Induction of lipogenesis in white fat during cold exposure in mice: link to lean phenotype

Popis výsledku anglicky

Futile substrate cycling based on lipolytic release of fatty acids (FA) from intracellular triacylglycerols (TAG) and their re-esterification (TAG/FA cycling), as well as de novo FA synthesis (de novo lipogenesis (DNL)), represent the core energy-consuming biochemical activities of white adipose tissue (WAT). We aimed to characterize their roles in cold-induced thermogenesis and energy homeostasis.Male obesity-resistant A/J and obesity-prone C57BL/6J mice maintained at 30 degrees C were exposed to 6 degrees C for 2 or 7 days. In epididymal WAT (eWAT), TAG synthesis and DNL were determined using in vivo H-2 incorporation from (H2O)-H-2 into tissue TAG and nuclear magnetic resonance spectroscopy. Quantitative real-time-PCR and/or immunohistochemistry and western blotting were used to determine the expression of selected genes and proteins in WAT and liver.The mass of WAT depots declined during cold exposure (CE). Plasma levels of TAG and non-esterified FA were decreased by day 2 but tended to normalize by day 7 of CE. TAG synthesis (reflecting TAG/FA cycle activity) gradually increased during CE. DNL decreased by day 2 of CE but increased several fold over the control values by day 7. Expression of genes involved in lipolysis, glyceroneogenesis, FA re-esterification, FA oxidation and mitochondrial biogenesis in eWAT was induced during CE. All these changes were more pronounced in obesity-resistant A/J than in B6 mice and occurred in the absence of uncoupling protein 1 in eWAT. Leptin and fibroblast growth factor 21 plasma levels were differentially affected by CE in the two mouse strains. Our results indicate integrated involvement of (i) TAG/FA cycling and DNL in WAT, and (ii) hepatic very-low-density lipoprotein-TAG synthesis in the control of blood lipid levels and provision of FA fuels for thermogenesis in cold. They suggest that lipogenesis in WAT contributes to a lean phenotype.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30202 - Endocrinology and metabolism (including diabetes, hormones)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Obesity

ISSN

0307-0565

e-ISSN

—

Svazek periodika

41

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

372-380

Kód UT WoS článku

000395816000003

EID výsledku v databázi Scopus

2-s2.0-85009827360