Plant and yeast cornichon possess a conserved acidic motif required for correct targeting of plasma membrane cargos
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F17%3A00477231" target="_blank" >RIV/67985823:_____/17:00477231 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.bbamcr.2017.07.004" target="_blank" >http://dx.doi.org/10.1016/j.bbamcr.2017.07.004</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bbamcr.2017.07.004" target="_blank" >10.1016/j.bbamcr.2017.07.004</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Plant and yeast cornichon possess a conserved acidic motif required for correct targeting of plasma membrane cargos
Popis výsledku v původním jazyce
The export of membrane proteins along the secretory pathway is initiated at the endoplasmic reticulum after proteins are folded and packaged inside this organelle by their recruiting into the coat complex COPII vesicles. It is proposed that cargo receptors are required for the correct transport of proteins to its target membrane, however, little is known about ER export signals for cargo receptors. Erv14/Comichon belong to a well conserved protein family in Eukaryotes, and have been proposed to function as cargo receptors for many trans membrane proteins. Amino acid sequence alignment showed the presence of a conserved acidic motif in the C terminal in homologues from plants and yeast. Here, we demonstrate that mutation of the C-terminal acidic motif from ScErv14 or OsCNIH1, did not alter the localization of these cargo receptors, however it modified the proper targeting of the plasma membrane transporters Nhalp, Pdrl2p and Qdr2p. Our results suggest that mistargeting of these plasma membrane proteins is a consequence of a weaker interaction between the cargo receptor and cargo proteins caused by the mutation of the C-terminal acidic motif.
Název v anglickém jazyce
Plant and yeast cornichon possess a conserved acidic motif required for correct targeting of plasma membrane cargos
Popis výsledku anglicky
The export of membrane proteins along the secretory pathway is initiated at the endoplasmic reticulum after proteins are folded and packaged inside this organelle by their recruiting into the coat complex COPII vesicles. It is proposed that cargo receptors are required for the correct transport of proteins to its target membrane, however, little is known about ER export signals for cargo receptors. Erv14/Comichon belong to a well conserved protein family in Eukaryotes, and have been proposed to function as cargo receptors for many trans membrane proteins. Amino acid sequence alignment showed the presence of a conserved acidic motif in the C terminal in homologues from plants and yeast. Here, we demonstrate that mutation of the C-terminal acidic motif from ScErv14 or OsCNIH1, did not alter the localization of these cargo receptors, however it modified the proper targeting of the plasma membrane transporters Nhalp, Pdrl2p and Qdr2p. Our results suggest that mistargeting of these plasma membrane proteins is a consequence of a weaker interaction between the cargo receptor and cargo proteins caused by the mutation of the C-terminal acidic motif.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Biochimica Et Biophysica Acta-Molecular Cell Research
ISSN
0167-4889
e-ISSN
—
Svazek periodika
1864
Číslo periodika v rámci svazku
10
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
10
Strana od-do
1809-1818
Kód UT WoS článku
000411168100024
EID výsledku v databázi Scopus
2-s2.0-85025624436