Valve interstitial cell culture: Production of mature type I collagen and precise detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F17%3A00477592" target="_blank" >RIV/67985823:_____/17:00477592 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11510/17:10359550 RIV/00023001:_____/17:00075981

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jemt.22886" target="_blank" >http://dx.doi.org/10.1002/jemt.22886</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jemt.22886" target="_blank" >10.1002/jemt.22886</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Valve interstitial cell culture: Production of mature type I collagen and precise detection

Popis výsledku v původním jazyce

Collagen often acts as an extracellular and intracellular marker for in vitro experiments, and its quality defines tissue constructs. To validate collagen detection techniques, cardiac valve interstitial cells were isolated from pigs and cultured under two different conditions, with and without ascorbic acid. The culture with ascorbic acid reached higher cell growth and collagen deposition, although the expression levels of collagen gene stayed similar to the culture without ascorbic acid. The fluorescent microscopy was positive for collagen fibers in both the cultures. Visualization of only extracellular collagen returned a higher correlation coefficient when comparing the immunolabeling and second harmonic generation microscopy images in the culture with ascorbic acid. Lastly, it was proved that the hydroxyproline strongly contributes to the second-order susceptibility tensor of collagen molecules, and therefore the second harmonic generation signal is impaired in the culture without ascorbic acid.

Název v anglickém jazyce

Valve interstitial cell culture: Production of mature type I collagen and precise detection

Popis výsledku anglicky

Collagen often acts as an extracellular and intracellular marker for in vitro experiments, and its quality defines tissue constructs. To validate collagen detection techniques, cardiac valve interstitial cells were isolated from pigs and cultured under two different conditions, with and without ascorbic acid. The culture with ascorbic acid reached higher cell growth and collagen deposition, although the expression levels of collagen gene stayed similar to the culture without ascorbic acid. The fluorescent microscopy was positive for collagen fibers in both the cultures. Visualization of only extracellular collagen returned a higher correlation coefficient when comparing the immunolabeling and second harmonic generation microscopy images in the culture with ascorbic acid. Lastly, it was proved that the hydroxyproline strongly contributes to the second-order susceptibility tensor of collagen molecules, and therefore the second harmonic generation signal is impaired in the culture without ascorbic acid.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy Research and Technique

ISSN

1059-910X

e-ISSN

—

Svazek periodika

80

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

936-942

Kód UT WoS článku

000406242500015

EID výsledku v databázi Scopus

2-s2.0-85018361386