Modification of Calcium Retention Capacity of Rat Liver Mitochondria by Phosphate and Tert-Butyl Hydroperoxide

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00504504" target="_blank" >RIV/67985823:_____/19:00504504 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/19:10393759

Výsledek na webu

<a href="https://www.biomed.cas.cz/physiolres/pdf/2019/68_59.pdf" target="_blank" >https://www.biomed.cas.cz/physiolres/pdf/2019/68_59.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.33549/physiolres.933912" target="_blank" >10.33549/physiolres.933912</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Modification of Calcium Retention Capacity of Rat Liver Mitochondria by Phosphate and Tert-Butyl Hydroperoxide

Popis výsledku v původním jazyce

By determining the calcium retention capacity (CRC) of rat liver mitochondria, we confirmed and extended previous observations describing the activation of mitochondrial swelling by phosphate and tert-butyl hydroperoxide (t-BHP). Using CRC measurements, we showed that both phosphate and t-BHP decrease the extent of calcium accumulation required for the full mitochondrial permeability transition pore (MPTP) opening to 35 % of control values and to only 15 % when both phosphate and t-BHP are present in the medium. When changes in fluorescence were evaluated at higher resolution, we observed that in the presence of cyclosporine A fluorescence values return after each Ca2+ addition to basal values obtained before the Ca2+ addition. This indicates that the MPTP remains closed. However, in the absence of cyclosporine A, the basal fluorescence after each Ca2+ addition continuously increased. This increase was potentiated both by phosphate and t-BHP until the moment when the concentration of intramitochondrial calcium required for the full opening of the MPTP was reached. We conclude that in the absence of cyclosporine A, the MPTP is slowly opened after each Ca2+ addition and that this rate of opening can be modified by various factors such as the composition of the media and the experimental protocol used.

Název v anglickém jazyce

Modification of Calcium Retention Capacity of Rat Liver Mitochondria by Phosphate and Tert-Butyl Hydroperoxide

Popis výsledku anglicky

By determining the calcium retention capacity (CRC) of rat liver mitochondria, we confirmed and extended previous observations describing the activation of mitochondrial swelling by phosphate and tert-butyl hydroperoxide (t-BHP). Using CRC measurements, we showed that both phosphate and t-BHP decrease the extent of calcium accumulation required for the full mitochondrial permeability transition pore (MPTP) opening to 35 % of control values and to only 15 % when both phosphate and t-BHP are present in the medium. When changes in fluorescence were evaluated at higher resolution, we observed that in the presence of cyclosporine A fluorescence values return after each Ca2+ addition to basal values obtained before the Ca2+ addition. This indicates that the MPTP remains closed. However, in the absence of cyclosporine A, the basal fluorescence after each Ca2+ addition continuously increased. This increase was potentiated both by phosphate and t-BHP until the moment when the concentration of intramitochondrial calcium required for the full opening of the MPTP was reached. We conclude that in the absence of cyclosporine A, the MPTP is slowly opened after each Ca2+ addition and that this rate of opening can be modified by various factors such as the composition of the media and the experimental protocol used.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30105 - Physiology (including cytology)

Projekt

<a href="/cs/project/LTC17044" target="_blank" >LTC17044: Jaterní mitochondrie – zaměření na vliv věku, životního stylu a prostředí</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

—

Svazek periodika

68

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

59-65

Kód UT WoS článku

000460617400006

EID výsledku v databázi Scopus

2-s2.0-85062586692