Alterations to the adhesion, growth and osteogenic differentiation of human osteoblast-like cells on nanofibrous polylactide scaffolds with diamond nanoparticles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00509198" target="_blank" >RIV/67985823:_____/19:00509198 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378271:_____/19:00509198

Výsledek na webu

<a href="https://doi.org/10.1016/j.diamond.2019.05.007" target="_blank" >https://doi.org/10.1016/j.diamond.2019.05.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.diamond.2019.05.007" target="_blank" >10.1016/j.diamond.2019.05.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Alterations to the adhesion, growth and osteogenic differentiation of human osteoblast-like cells on nanofibrous polylactide scaffolds with diamond nanoparticles

Popis výsledku v původním jazyce

Nanofibrous scaffolds are promising materials for engineering various types of tissues, including bone tissue. The properties of nanofibers for bone tissue engineering can be improved by adding ceramic or carbon nanoparticles, including diamond nanoparticles (DNPs). In this study, poly(L-lactide) (PLLA) nanofibrous membranes reinforced with detonation DNPs (concentration from 0 to 12.28 wt%), provided by the NanoCarbon Research Institute (Japan) under the name NanoAmando (R), were prepared by a needle-less electrospinning process. The biocompatibility of these DNP-reinforced PLLA membranes was tested in vitro using two human lines of osteoblast-like cells, MG-63 and Saos-2. Microscopic and biochemical methods were used. In addition, the expression of cell survival markers, focal adhesion proteins and osteogenic markers were studied in both cell types at mRNA level and at protein level. The mitochondrial activity of both cells types decreased with increasing DNP concentration in the PLLA matrix. At the same time, the expression of cyclin D and survivin, i.e. factors supporting cell growth and survival were downregulated on the DNP-loaded PLLA scaffolds, while KLF6, an anti-proliferative factor, was often upregulated, at least in MG-63 cells. Thus, loading the nanofibrous polymeric scaffolds with detonation DNPs had rather adverse effects on the behavior of human bone-derived cell lines. These results are mostly in conflict with our earlier studies on poly(lactide-co-glycolide) (PLGA) scaffolds, loaded with DNPs prepared by etching the diamond layer fabricated by radiofrequency plasma-assisted chemical vapour deposition. The cell behavior on DNP-loaded scaffolds is therefore markedly influenced by the origin and the properties of the DNPs.

Název v anglickém jazyce

Alterations to the adhesion, growth and osteogenic differentiation of human osteoblast-like cells on nanofibrous polylactide scaffolds with diamond nanoparticles

Popis výsledku anglicky

Nanofibrous scaffolds are promising materials for engineering various types of tissues, including bone tissue. The properties of nanofibers for bone tissue engineering can be improved by adding ceramic or carbon nanoparticles, including diamond nanoparticles (DNPs). In this study, poly(L-lactide) (PLLA) nanofibrous membranes reinforced with detonation DNPs (concentration from 0 to 12.28 wt%), provided by the NanoCarbon Research Institute (Japan) under the name NanoAmando (R), were prepared by a needle-less electrospinning process. The biocompatibility of these DNP-reinforced PLLA membranes was tested in vitro using two human lines of osteoblast-like cells, MG-63 and Saos-2. Microscopic and biochemical methods were used. In addition, the expression of cell survival markers, focal adhesion proteins and osteogenic markers were studied in both cell types at mRNA level and at protein level. The mitochondrial activity of both cells types decreased with increasing DNP concentration in the PLLA matrix. At the same time, the expression of cyclin D and survivin, i.e. factors supporting cell growth and survival were downregulated on the DNP-loaded PLLA scaffolds, while KLF6, an anti-proliferative factor, was often upregulated, at least in MG-63 cells. Thus, loading the nanofibrous polymeric scaffolds with detonation DNPs had rather adverse effects on the behavior of human bone-derived cell lines. These results are mostly in conflict with our earlier studies on poly(lactide-co-glycolide) (PLGA) scaffolds, loaded with DNPs prepared by etching the diamond layer fabricated by radiofrequency plasma-assisted chemical vapour deposition. The cell behavior on DNP-loaded scaffolds is therefore markedly influenced by the origin and the properties of the DNPs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)

Projekt

<a href="/cs/project/GBP108%2F12%2FG108" target="_blank" >GBP108/12/G108: Příprava, modifikace a charakterizace materiálů zářením</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Diamond and Related Materials

ISSN

0925-9635

e-ISSN

—

Svazek periodika

97

Číslo periodika v rámci svazku

Aug

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

UNSP 107421

Kód UT WoS článku

000486134300023

EID výsledku v databázi Scopus

2-s2.0-85067289358