Effects of aging and tumorigenesis on coupling between the circadian clock and cell cycle in colonic mucosa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F20%3A00533406" target="_blank" >RIV/67985823:_____/20:00533406 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/20:10416766

Výsledek na webu

<a href="https://doi.org/10.1016/j.mad.2020.111317" target="_blank" >https://doi.org/10.1016/j.mad.2020.111317</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mad.2020.111317" target="_blank" >10.1016/j.mad.2020.111317</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of aging and tumorigenesis on coupling between the circadian clock and cell cycle in colonic mucosa

Popis výsledku v původním jazyce

Aging and tumorigenesis are associated with decline and disruption of circadian rhythms in many tissues and accumulating evidence indicates molecular link between circadian clock and cell cycle. The aim of this study was to investigate the effect of aging and tumorigenesis on coupling between cell cycle and circadian clock oscillators in colon, which undergoes regular rhythmicity of cell cycle and expresses peripheral circadian clock. Using healthy 14-week-old mice and 33-week-old mice with and without colorectal tumors, we showed that the 24-h expression profiles of clock genes and clock-controlled genes were mostly unaffected by aging, whereas the genes of cell cycle and cell proliferation were rhythmic in the young colons but were silenced during aging. On the other hand, tumorigenesis completely silenced or dampened the circadian rhythmicity of the clock genes but only a few genes associated with cell cycle progression and cell proliferation. These results suggest that aging impacts the colonic circadian clock moderately but markedly suppresses the rhythms of cell cycle genes and appears to uncouple the cell cycle machinery from circadian clock control. Conversely, tumorigenesis predominantly affects the rhythms of colonic circadian clocks but is not associated with uncoupling of circadian clock and cell cycle.

Název v anglickém jazyce

Effects of aging and tumorigenesis on coupling between the circadian clock and cell cycle in colonic mucosa

Popis výsledku anglicky

Aging and tumorigenesis are associated with decline and disruption of circadian rhythms in many tissues and accumulating evidence indicates molecular link between circadian clock and cell cycle. The aim of this study was to investigate the effect of aging and tumorigenesis on coupling between cell cycle and circadian clock oscillators in colon, which undergoes regular rhythmicity of cell cycle and expresses peripheral circadian clock. Using healthy 14-week-old mice and 33-week-old mice with and without colorectal tumors, we showed that the 24-h expression profiles of clock genes and clock-controlled genes were mostly unaffected by aging, whereas the genes of cell cycle and cell proliferation were rhythmic in the young colons but were silenced during aging. On the other hand, tumorigenesis completely silenced or dampened the circadian rhythmicity of the clock genes but only a few genes associated with cell cycle progression and cell proliferation. These results suggest that aging impacts the colonic circadian clock moderately but markedly suppresses the rhythms of cell cycle genes and appears to uncouple the cell cycle machinery from circadian clock control. Conversely, tumorigenesis predominantly affects the rhythms of colonic circadian clocks but is not associated with uncoupling of circadian clock and cell cycle.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30105 - Physiology (including cytology)

Projekt

<a href="/cs/project/GA13-08304S" target="_blank" >GA13-08304S: Úloha vnitřního časovacího systému v patogenezi kolorektálního karcinomu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mechanisms of Ageing and Development

ISSN

0047-6374

e-ISSN

—

Svazek periodika

190

Číslo periodika v rámci svazku

Sep

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

10

Strana od-do

111317

Kód UT WoS článku

000569392800005

EID výsledku v databázi Scopus

2-s2.0-85089350888