Communicating macropores in PHEMA-based hydrogels for cell seeding: probabilistic open pore simulation and direct micro-CT proof

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F21%3A00535213" target="_blank" >RIV/67985823:_____/21:00535213 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21220/21:00344096 RIV/61389013:_____/21:00535213 RIV/00216208:11110/21:10421919 RIV/00064165:_____/21:10421919

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0264127520308480?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0264127520308480?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.matdes.2020.109312" target="_blank" >10.1016/j.matdes.2020.109312</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Communicating macropores in PHEMA-based hydrogels for cell seeding: probabilistic open pore simulation and direct micro-CT proof

Popis výsledku v původním jazyce

Open macroporosity is crucial for scaffolds in tissue engineering. Porogen-templating method is an attractive approach for fabrication of macroporous hydrogels, however, the effect of shape and amount of template particles on imprinted structure has not yet been quantitatively established. We present a mathematical model for simulating the formation of paths percolating through distributed cubical particles as a function of the filling volume. The model was used to select the fraction of NaCl particles as templates for preparation of hydrogels with communicating pores. Hydrogels were prepared from 2-hydroxyethyl methacrylate (HEMA) copolymerized with 2-ethoxyethyl methacrylate (EOEMA), [2-methacryloyloxy)ethyl]trimethylammonium chloride (MOETACl) or ionizable methacrylic acid (MANa) to modulate swelling, surface and mechanical properties of gels. Micro-CT analysis of swollen samples proved a highly-interconnected pore structure. Charged hydrogels swelled more and their apparent elastic modulus G′ was below 1 kPa. For PHEMA and P(HEMA/EOEMA) hydrogels, G′ was 5 and 80 kPa, respectively. Within two-week in vitro studies, MG63 osteoblasts proliferated fastest on P(HEMA/EOEMA) showing the lowest swelling and the highest elastic modulus, whereas cell growth was impaired on positively charged P(HEMA/MOETACl). The mathematical simulation of cubical particle packing in hydrogels and micro-CT data in swollen state provided evidence of an extensive void communication in 3D.

Název v anglickém jazyce

Communicating macropores in PHEMA-based hydrogels for cell seeding: probabilistic open pore simulation and direct micro-CT proof

Popis výsledku anglicky

Open macroporosity is crucial for scaffolds in tissue engineering. Porogen-templating method is an attractive approach for fabrication of macroporous hydrogels, however, the effect of shape and amount of template particles on imprinted structure has not yet been quantitatively established. We present a mathematical model for simulating the formation of paths percolating through distributed cubical particles as a function of the filling volume. The model was used to select the fraction of NaCl particles as templates for preparation of hydrogels with communicating pores. Hydrogels were prepared from 2-hydroxyethyl methacrylate (HEMA) copolymerized with 2-ethoxyethyl methacrylate (EOEMA), [2-methacryloyloxy)ethyl]trimethylammonium chloride (MOETACl) or ionizable methacrylic acid (MANa) to modulate swelling, surface and mechanical properties of gels. Micro-CT analysis of swollen samples proved a highly-interconnected pore structure. Charged hydrogels swelled more and their apparent elastic modulus G′ was below 1 kPa. For PHEMA and P(HEMA/EOEMA) hydrogels, G′ was 5 and 80 kPa, respectively. Within two-week in vitro studies, MG63 osteoblasts proliferated fastest on P(HEMA/EOEMA) showing the lowest swelling and the highest elastic modulus, whereas cell growth was impaired on positively charged P(HEMA/MOETACl). The mathematical simulation of cubical particle packing in hydrogels and micro-CT data in swollen state provided evidence of an extensive void communication in 3D.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials and Design

ISSN

0264-1275

e-ISSN

1873-4197

Svazek periodika

198

Číslo periodika v rámci svazku

15 January

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

17

Strana od-do

109312

Kód UT WoS článku

000607549100007

EID výsledku v databázi Scopus

2-s2.0-85097233064