Colloidally stable P(DMA-AGME)-Ale-coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ nanoparticles as a multimodal contrast agent for down- and upconversion luminescence, magnetic resonance imaging, and computed tomography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F21%3A00537739" target="_blank" >RIV/67985823:_____/21:00537739 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/21:00537739 RIV/00216208:11110/21:10422721

Výsledek na webu

<a href="https://www.mdpi.com/2079-4991/11/1/230" target="_blank" >https://www.mdpi.com/2079-4991/11/1/230</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/nano11010230" target="_blank" >10.3390/nano11010230</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Colloidally stable P(DMA-AGME)-Ale-coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ nanoparticles as a multimodal contrast agent for down- and upconversion luminescence, magnetic resonance imaging, and computed tomography

Popis výsledku v původním jazyce

Multimodal imaging, integrating several modalities including down- and up-conversion luminescence, T1- and T2(T2*)-weighted MRI, and CT contrasting in one system, is very promising for improved diagnosis of severe medical disorders. To reach the goal, it is necessary to develop suitable nanoparticles that are highly colloidally stable in biologically relevant media. Here, hydrophilic poly(N,N-dimethylacrylamide-N-acryloylglycine methyl ester)-alendronate-[P(DMA-AGME)-Ale]-coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ nanoparticles were synthesized by a coprecipitation method in ethylene glycol (EG) followed by coating with the polymer. The particles were tho-roughly characterized by a dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray energy dispersive spectroscopy (EDAX), selected area electron diffraction (SAED), elemental ana-lysis and fluorescence spectroscopy. Aqueous particle dispersions exhibited excellent colloidal stability in water and physiological buffers. In vitro toxicity assessments suggested no or only mild toxicity of the surface-engineered Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ particles in a wide range of concentrations. Internalization of the particles by several types of cells, including HeLa, HF, HepG2, and INS, was confirmed by a down- and up-conversion confocal microscopy. Newly developed particles thus proved to be an efficient contrast agent for fluorescence imaging, T1- and T2(T2*)-weighted magnetic resonance imaging (MRI), and computed tomography (CT).

Název v anglickém jazyce

Colloidally stable P(DMA-AGME)-Ale-coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ nanoparticles as a multimodal contrast agent for down- and upconversion luminescence, magnetic resonance imaging, and computed tomography

Popis výsledku anglicky

Multimodal imaging, integrating several modalities including down- and up-conversion luminescence, T1- and T2(T2*)-weighted MRI, and CT contrasting in one system, is very promising for improved diagnosis of severe medical disorders. To reach the goal, it is necessary to develop suitable nanoparticles that are highly colloidally stable in biologically relevant media. Here, hydrophilic poly(N,N-dimethylacrylamide-N-acryloylglycine methyl ester)-alendronate-[P(DMA-AGME)-Ale]-coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ nanoparticles were synthesized by a coprecipitation method in ethylene glycol (EG) followed by coating with the polymer. The particles were tho-roughly characterized by a dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray energy dispersive spectroscopy (EDAX), selected area electron diffraction (SAED), elemental ana-lysis and fluorescence spectroscopy. Aqueous particle dispersions exhibited excellent colloidal stability in water and physiological buffers. In vitro toxicity assessments suggested no or only mild toxicity of the surface-engineered Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ particles in a wide range of concentrations. Internalization of the particles by several types of cells, including HeLa, HF, HepG2, and INS, was confirmed by a down- and up-conversion confocal microscopy. Newly developed particles thus proved to be an efficient contrast agent for fluorescence imaging, T1- and T2(T2*)-weighted magnetic resonance imaging (MRI), and computed tomography (CT).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nanomaterials

ISSN

2079-4991

e-ISSN

2079-4991

Svazek periodika

11

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

21

Strana od-do

230

Kód UT WoS článku

000610663900001

EID výsledku v databázi Scopus

2-s2.0-85100140596