Growth Factors VEGF-A(165) and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F21%3A00541641" target="_blank" >RIV/67985823:_____/21:00541641 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/21:00541641

Výsledek na webu

<a href="https://www.mdpi.com/1422-0067/22/4/1843" target="_blank" >https://www.mdpi.com/1422-0067/22/4/1843</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms22041843" target="_blank" >10.3390/ijms22041843</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Growth Factors VEGF-A(165) and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation

Popis výsledku v původním jazyce

Vascular endothelial growth factor-A(165) (VEGF-A(165)) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A(165) and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A(165) stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinant growth factors was slightly lower than that of commercially available factors, which was attributed to the presence of some impurities. The stimulatory effect of the VEGF-A(165) on cell adhesion was rather weak, especially in ADSCs. FGF-2 was a potent stimulator of the adhesion of ADSCs but had no to negative effect on the adhesion of HUVECs. In sum, FGF-2 and VEGF-A(165) have diverse effects on the behavior of different cell types, which maybe utilized in tissue engineering.

Název v anglickém jazyce

Growth Factors VEGF-A(165) and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation

Popis výsledku anglicky

Vascular endothelial growth factor-A(165) (VEGF-A(165)) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A(165) and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A(165) stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinant growth factors was slightly lower than that of commercially available factors, which was attributed to the presence of some impurities. The stimulatory effect of the VEGF-A(165) on cell adhesion was rather weak, especially in ADSCs. FGF-2 was a potent stimulator of the adhesion of ADSCs but had no to negative effect on the adhesion of HUVECs. In sum, FGF-2 and VEGF-A(165) have diverse effects on the behavior of different cell types, which maybe utilized in tissue engineering.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30404 - Biomaterials (as related to medical implants, devices, sensors)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

1422-0067

Svazek periodika

22

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

27

Strana od-do

1843

Kód UT WoS článku

000623786800001

EID výsledku v databázi Scopus

2-s2.0-85100662467