Human osteoblast-like SAOS-2 cells on submicron-scale fibers coated with nanocrystalline diamond films

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F21%3A00541653" target="_blank" >RIV/67985823:_____/21:00541653 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24410/21:00008427 RIV/46747885:24620/21:00008427 RIV/68378271:_____/21:00541653 RIV/68407700:21110/21:00345278 RIV/68407700:21460/21:00345278

Výsledek na webu

<a href="https://doi.org/10.1016/j.msec.2020.111792" target="_blank" >https://doi.org/10.1016/j.msec.2020.111792</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.msec.2020.111792" target="_blank" >10.1016/j.msec.2020.111792</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Human osteoblast-like SAOS-2 cells on submicron-scale fibers coated with nanocrystalline diamond films

Popis výsledku v původním jazyce

A unique composite nanodiamond-based porous material with a hierarchically-organized submicron-nanostructure was constructed for potential bone tissue engineering. This material consisted of submicron fibers prepared by electrospinning of silicon oxide (SiOx), which were oxygen-terminated (O-SiOx) and were hermetically coated with nanocrystalline diamond (NCD) films. The NCD films were then terminated with hydrogen (HNCD) or oxygen (O-NCD). The materials were tested as substrates for the adhesion, growth and osteogenic differentiation of human osteoblast-like Saos-2 cells. The number and the spreading area of the initially adhered cells, their growth rate during 7 days after seeding and the activity of alkaline phosphatase (ALP) were significantly higher on the NCD-coated samples than on the uncoated 0-SiO x samples. In addition, the concentration of type I collagen was significantly higher in the cells on the O-NCD-coated samples than on the bare O-SiOx samples. The observed differences could be attributed to the tunable wettability of NCD and to the more appropriate surface morphology of the NCD-coated samples in contrast to the less stable, rapidly eroding bare SiOx surface. The H-NCD coatings and the O-NCD coatings both promoted similar initial adhesion of Saos-2 cells, but the subsequent cell proliferation activity was higher on the O-NCD-coated samples. The concentration of beta-actin, vinculin, type I collagen and alkaline phosphatase (ALP), the ALP activity, and also the calcium deposition tended to be higher in the cells on the O-NCD-coated samples than on the H-NCD-coated samples, although these differences did not reach statistical significance. The improved cell performance on the O-NCD-coated samples could be attributed to higher wettability of these samples (water drop contact angle less than 10 degrees), while the H-NCD-coated samples were hydrophobic (contact angle >701. NCD-coated porous SiOx meshes can therefore be considered as appropriate scaffolds for bone tissue engineering, particularly those with an O-terminated NCD coating.

Název v anglickém jazyce

Human osteoblast-like SAOS-2 cells on submicron-scale fibers coated with nanocrystalline diamond films

Popis výsledku anglicky

A unique composite nanodiamond-based porous material with a hierarchically-organized submicron-nanostructure was constructed for potential bone tissue engineering. This material consisted of submicron fibers prepared by electrospinning of silicon oxide (SiOx), which were oxygen-terminated (O-SiOx) and were hermetically coated with nanocrystalline diamond (NCD) films. The NCD films were then terminated with hydrogen (HNCD) or oxygen (O-NCD). The materials were tested as substrates for the adhesion, growth and osteogenic differentiation of human osteoblast-like Saos-2 cells. The number and the spreading area of the initially adhered cells, their growth rate during 7 days after seeding and the activity of alkaline phosphatase (ALP) were significantly higher on the NCD-coated samples than on the uncoated 0-SiO x samples. In addition, the concentration of type I collagen was significantly higher in the cells on the O-NCD-coated samples than on the bare O-SiOx samples. The observed differences could be attributed to the tunable wettability of NCD and to the more appropriate surface morphology of the NCD-coated samples in contrast to the less stable, rapidly eroding bare SiOx surface. The H-NCD coatings and the O-NCD coatings both promoted similar initial adhesion of Saos-2 cells, but the subsequent cell proliferation activity was higher on the O-NCD-coated samples. The concentration of beta-actin, vinculin, type I collagen and alkaline phosphatase (ALP), the ALP activity, and also the calcium deposition tended to be higher in the cells on the O-NCD-coated samples than on the H-NCD-coated samples, although these differences did not reach statistical significance. The improved cell performance on the O-NCD-coated samples could be attributed to higher wettability of these samples (water drop contact angle less than 10 degrees), while the H-NCD-coated samples were hydrophobic (contact angle >701. NCD-coated porous SiOx meshes can therefore be considered as appropriate scaffolds for bone tissue engineering, particularly those with an O-terminated NCD coating.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30404 - Biomaterials (as related to medical implants, devices, sensors)

Projekt

<a href="/cs/project/NV19-02-00068" target="_blank" >NV19-02-00068: Bioartificiální kardiovaskulární záplaty a cévní náhrady na bázi porcinního kolagenu zesílené nano/mikrovlákny remodelované pomocí kmenových buněk v bioreaktorech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials Science & Engineering C-Materials for Biological Applications

ISSN

0928-4931

e-ISSN

1873-0191

Svazek periodika

121

Číslo periodika v rámci svazku

Feb

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

15

Strana od-do

111792

Kód UT WoS článku

000619124600007

EID výsledku v databázi Scopus

2-s2.0-85098711683