Modification of heat-induced whey protein isolate hydrogel with highly bioactive glass particles results in promising biomaterial for bone tissue engineering
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F21%3A00542895" target="_blank" >RIV/67985823:_____/21:00542895 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/46747885:24510/21:00009631
Výsledek na webu
<a href="https://doi.org/10.1016/j.matdes.2021.109749" target="_blank" >https://doi.org/10.1016/j.matdes.2021.109749</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.matdes.2021.109749" target="_blank" >10.1016/j.matdes.2021.109749</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Modification of heat-induced whey protein isolate hydrogel with highly bioactive glass particles results in promising biomaterial for bone tissue engineering
Popis výsledku v původním jazyce
This study deals with the design and comprehensive evaluation of novel hydrogels based on whey protein isolate (WPI) for tissue regeneration. So far, WPI has been considered mainly as a food industry by-product and there are very few reports on the application of WPI in tissue engineering (TE). In this work, WPI-based hydrogels were modified with bioactive glass (BG), which is commonly used as a bone substitute material. Ready-to-use, sterile hydrogels were produced by a simple technique, namely heat-induced gelation. Two different concentrations (10 and 20% w/w) of sol–gel-derived BG particles of two different sizes (2.5 and <45 µm) were compared. µCT analysis showed that hydrogels were highly porous with almost 100% pore interconnectivity. BG particles were generally homogenously distributed in the hydrogel matrix, affecting pore size, and reducing material porosity. Thermal analysis showed that the presence of BG particles in WPI matrix reduced water content in hydrogels and improved their thermal stability. BG particles decreased enzymatic degradation of the materials. The materials underwent mineralization in simulated biological fluids (PBS and SBF) and possessed high radical scavenging capacity. In vitro tests indicated that hydrogels were cytocompatible and supported MG-63 osteoblastic cell functions.
Název v anglickém jazyce
Modification of heat-induced whey protein isolate hydrogel with highly bioactive glass particles results in promising biomaterial for bone tissue engineering
Popis výsledku anglicky
This study deals with the design and comprehensive evaluation of novel hydrogels based on whey protein isolate (WPI) for tissue regeneration. So far, WPI has been considered mainly as a food industry by-product and there are very few reports on the application of WPI in tissue engineering (TE). In this work, WPI-based hydrogels were modified with bioactive glass (BG), which is commonly used as a bone substitute material. Ready-to-use, sterile hydrogels were produced by a simple technique, namely heat-induced gelation. Two different concentrations (10 and 20% w/w) of sol–gel-derived BG particles of two different sizes (2.5 and <45 µm) were compared. µCT analysis showed that hydrogels were highly porous with almost 100% pore interconnectivity. BG particles were generally homogenously distributed in the hydrogel matrix, affecting pore size, and reducing material porosity. Thermal analysis showed that the presence of BG particles in WPI matrix reduced water content in hydrogels and improved their thermal stability. BG particles decreased enzymatic degradation of the materials. The materials underwent mineralization in simulated biological fluids (PBS and SBF) and possessed high radical scavenging capacity. In vitro tests indicated that hydrogels were cytocompatible and supported MG-63 osteoblastic cell functions.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
Návaznosti výsledku
Projekt
<a href="/cs/project/GA20-01570S" target="_blank" >GA20-01570S: Zlepšení osteointegrace kostních implantátů jejich pokrytím feroelektrickými vrstvami</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Materials and Design
ISSN
0264-1275
e-ISSN
1873-4197
Svazek periodika
205
Číslo periodika v rámci svazku
July
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
14
Strana od-do
109749
Kód UT WoS článku
000663557400007
EID výsledku v databázi Scopus
2-s2.0-85105033699