Biomimetic biphasic curdlan-based scaffold for osteochondral tissue engineering applications Characterization and preliminary evaluation of mesenchymal stem cell response in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F22%3A00558807" target="_blank" >RIV/67985823:_____/22:00558807 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.bioadv.2022.212724" target="_blank" >https://doi.org/10.1016/j.bioadv.2022.212724</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bioadv.2022.212724" target="_blank" >10.1016/j.bioadv.2022.212724</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biomimetic biphasic curdlan-based scaffold for osteochondral tissue engineering applications Characterization and preliminary evaluation of mesenchymal stem cell response in vitro

Popis výsledku v původním jazyce

Osteochondral defects remain a huge problem in medicine today. Biomimetic bi- or multi-phasic scaffolds constitute a very promising alternative to osteochondral autografts and allografts. In this study, a new curdlanbased scaffold was designed for osteochondral tissue engineering applications. To achieve biomimetic properties, it was enriched with a protein component ??? whey protein isolate as well as a ceramic ingredient ??? hydroxyapatite granules. The scaffold was fabricated via a simple and cost-efficient method, which represents a significant advantage. Importantly, this technique allowed generation of a scaffold with two distinct, but integrated phases. Scanning electron microcopy and optical profilometry observations demonstrated that phases of biomaterial possessed different structural properties. The top layer of the biomaterial (mimicking the cartilage) was smoother than the bottom one (mimicking the subchondral bone), which is beneficial from a biological point of view because unlike bone, cartilage is a smooth tissue. Moreover, mechanical testing showed that the top layer of the biomaterial had mechanical properties close to those of natural cartilage. Although the mechanical properties of the bottom layer of scaffold were lower than those of the subchondral bone, it was still higher than in many analogous systems. Most importantly, cell culture experiments indicated that the biomaterial possessed high cytocompatibility towards adipose tissue-derived mesenchymal stem cells and bone marrow-derived mesenchymal stem cells in vitro. Both phases of the scaffold enhanced cell adhesion, proliferation, and chondrogenic differentiation of stem cells (revealing its chondroinductive properties in vitro) as well as osteogenic differentiation of these cells (revealing its osteoinductive properties in vitro). Given all features of the novel curdlan-based scaffold, it is worth noting that it may be considered as promising candidate for osteochondral tissue engineering applications.

Název v anglickém jazyce

Biomimetic biphasic curdlan-based scaffold for osteochondral tissue engineering applications Characterization and preliminary evaluation of mesenchymal stem cell response in vitro

Popis výsledku anglicky

Osteochondral defects remain a huge problem in medicine today. Biomimetic bi- or multi-phasic scaffolds constitute a very promising alternative to osteochondral autografts and allografts. In this study, a new curdlanbased scaffold was designed for osteochondral tissue engineering applications. To achieve biomimetic properties, it was enriched with a protein component ??? whey protein isolate as well as a ceramic ingredient ??? hydroxyapatite granules. The scaffold was fabricated via a simple and cost-efficient method, which represents a significant advantage. Importantly, this technique allowed generation of a scaffold with two distinct, but integrated phases. Scanning electron microcopy and optical profilometry observations demonstrated that phases of biomaterial possessed different structural properties. The top layer of the biomaterial (mimicking the cartilage) was smoother than the bottom one (mimicking the subchondral bone), which is beneficial from a biological point of view because unlike bone, cartilage is a smooth tissue. Moreover, mechanical testing showed that the top layer of the biomaterial had mechanical properties close to those of natural cartilage. Although the mechanical properties of the bottom layer of scaffold were lower than those of the subchondral bone, it was still higher than in many analogous systems. Most importantly, cell culture experiments indicated that the biomaterial possessed high cytocompatibility towards adipose tissue-derived mesenchymal stem cells and bone marrow-derived mesenchymal stem cells in vitro. Both phases of the scaffold enhanced cell adhesion, proliferation, and chondrogenic differentiation of stem cells (revealing its chondroinductive properties in vitro) as well as osteogenic differentiation of these cells (revealing its osteoinductive properties in vitro). Given all features of the novel curdlan-based scaffold, it is worth noting that it may be considered as promising candidate for osteochondral tissue engineering applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30404 - Biomaterials (as related to medical implants, devices, sensors)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomaterials Advances

ISSN

2772-9508

e-ISSN

2772-9508

Svazek periodika

135

Číslo periodika v rámci svazku

April

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

19

Strana od-do

212724

Kód UT WoS článku

000812231700001

EID výsledku v databázi Scopus

2-s2.0-85131954631