CD36 regulates substrates utilisation in brown adipose tissue of spontaneously hypertensive rats: In vitro study
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F23%3A00571373" target="_blank" >RIV/67985823:_____/23:00571373 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00023001:_____/23:00083949 RIV/00216208:11310/23:10465899 RIV/00216208:11110/23:10465899
Výsledek na webu
<a href="https://doi.org/10.1371/journal.pone.0283276" target="_blank" >https://doi.org/10.1371/journal.pone.0283276</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0283276" target="_blank" >10.1371/journal.pone.0283276</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
CD36 regulates substrates utilisation in brown adipose tissue of spontaneously hypertensive rats: In vitro study
Popis výsledku v původním jazyce
Thermogenesis in brown adipose tissue (BAT) uses intracellular triglycerides, circulating free fatty acids and glucose as the main substrates. The objective of the current study was to analyse the role of CD36 fatty acid translocase in regulation of glucose and fatty acid utilisation in BAT. BAT isolated from spontaneously hypertensive rat (SHR) with mutant Cd36 gene and SHR-Cd36 transgenic rats with wild type variant was incubated in media containing labeled glucose and palmitate to measure substrate incorporation and oxidation. SHR-Cd36 versus SHR rats showed significantly increased glucose incorporation into intracellular lipids associated with reduced glycogen synthase kinase 3β (GSK-3β) protein expression and phosphorylation and increased oxidation of exogenous palmitate. It can be concluded that CD36 enhances glucose transport for lipogenesis in BAT by suppressing GSK-3β and promotes direct palmitate oxidation.
Název v anglickém jazyce
CD36 regulates substrates utilisation in brown adipose tissue of spontaneously hypertensive rats: In vitro study
Popis výsledku anglicky
Thermogenesis in brown adipose tissue (BAT) uses intracellular triglycerides, circulating free fatty acids and glucose as the main substrates. The objective of the current study was to analyse the role of CD36 fatty acid translocase in regulation of glucose and fatty acid utilisation in BAT. BAT isolated from spontaneously hypertensive rat (SHR) with mutant Cd36 gene and SHR-Cd36 transgenic rats with wild type variant was incubated in media containing labeled glucose and palmitate to measure substrate incorporation and oxidation. SHR-Cd36 versus SHR rats showed significantly increased glucose incorporation into intracellular lipids associated with reduced glycogen synthase kinase 3β (GSK-3β) protein expression and phosphorylation and increased oxidation of exogenous palmitate. It can be concluded that CD36 enhances glucose transport for lipogenesis in BAT by suppressing GSK-3β and promotes direct palmitate oxidation.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30105 - Physiology (including cytology)
Návaznosti výsledku
Projekt
<a href="/cs/project/LX22NPO5104" target="_blank" >LX22NPO5104: Národní institut pro výzkum metabolických a kardiovaskulárních onemocnění</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
PLoS ONE
ISSN
1932-6203
e-ISSN
1932-6203
Svazek periodika
18
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
11
Strana od-do
e0283276
Kód UT WoS článku
000970963500038
EID výsledku v databázi Scopus
2-s2.0-85152599418