Status epilepticus‑induced neuronal degeneration in the immature rat zona incerta is confined to its rostral sector

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F25%3A00619559" target="_blank" >RIV/67985823:_____/25:00619559 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/25:10497320 RIV/00216208:11130/25:10497320

Výsledek na webu

<a href="https://doi.org/10.55782/ane-2024-2447" target="_blank" >https://doi.org/10.55782/ane-2024-2447</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.55782/ane-2024-2447" target="_blank" >10.55782/ane-2024-2447</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Status epilepticus‑induced neuronal degeneration in the immature rat zona incerta is confined to its rostral sector

Popis výsledku v původním jazyce

The distribution and morphology of neuronal degeneration were observed and analyzed in each sector of the zona incerta in a lithium-pilocarpine (LiCl) Wistar rat model of status epilepticus in 12, 15, 18, 21, and 25-day-old rats and survival intervals of 4, 8, 12, 24, and 48 hours. Status epilepticus was induced via intraperitoneal (IP) injection of LiCl (3 mmol/kg) 24 hours before an injection of pilocarpine (40 mg/kg, IP). Motor seizures were suppressed by paraldehyde (0.3-0.6 ml/kg, IP) two hours after status epilepticus onset. Animals were anesthetized using urethane and perfused with phosphate-buffered saline followed by 4% paraformaldehyde. Brains were sectioned and Nissl stained for map guidance, with fluoro-Jade B fluorescence used to detect degenerated neurons. Fluoro-jade B-positive neurons were plotted to a standard stereotaxic atlas, their distribution was quantified, and their long-axis diameter was measured. Fluoro-jade B-positive neurons were found in pups aged 15 days and older 24 hours after status epilepticus, in which their numbers increased, and their perikaryon size decreased with advancing age. Thus, neuronal damage severity was dependent on age and survival interval. Neuronal damage was only found in the rostral sector of the zona incerta, a region that exhibits a small number of inhibitory neurons and is reciprocally connected to the limbic cortex. This system of hyperactivity, coupled with inhibitory neurons, may be the underlying cause of the neuronal degeneration and explain why it was confined to the rostral sector of the zona incerta.

Název v anglickém jazyce

Status epilepticus‑induced neuronal degeneration in the immature rat zona incerta is confined to its rostral sector

Popis výsledku anglicky

The distribution and morphology of neuronal degeneration were observed and analyzed in each sector of the zona incerta in a lithium-pilocarpine (LiCl) Wistar rat model of status epilepticus in 12, 15, 18, 21, and 25-day-old rats and survival intervals of 4, 8, 12, 24, and 48 hours. Status epilepticus was induced via intraperitoneal (IP) injection of LiCl (3 mmol/kg) 24 hours before an injection of pilocarpine (40 mg/kg, IP). Motor seizures were suppressed by paraldehyde (0.3-0.6 ml/kg, IP) two hours after status epilepticus onset. Animals were anesthetized using urethane and perfused with phosphate-buffered saline followed by 4% paraformaldehyde. Brains were sectioned and Nissl stained for map guidance, with fluoro-Jade B fluorescence used to detect degenerated neurons. Fluoro-jade B-positive neurons were plotted to a standard stereotaxic atlas, their distribution was quantified, and their long-axis diameter was measured. Fluoro-jade B-positive neurons were found in pups aged 15 days and older 24 hours after status epilepticus, in which their numbers increased, and their perikaryon size decreased with advancing age. Thus, neuronal damage severity was dependent on age and survival interval. Neuronal damage was only found in the rostral sector of the zona incerta, a region that exhibits a small number of inhibitory neurons and is reciprocally connected to the limbic cortex. This system of hyperactivity, coupled with inhibitory neurons, may be the underlying cause of the neuronal degeneration and explain why it was confined to the rostral sector of the zona incerta.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Neurobiologiae Experimentalis

ISSN

0065-1400

e-ISSN

1689-0035

Svazek periodika

85

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

11

Strana od-do

38-48

Kód UT WoS článku

001473390800004

EID výsledku v databázi Scopus

2-s2.0-105003706697