3D Microstructural Characterization of Human Deep Fascia Using Optical Projection Tomography, Digital Light Sheet Microscopy, and Magnetic Resonance Microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F25%3A00640547" target="_blank" >RIV/67985823:_____/25:00640547 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1002/jemt.70035" target="_blank" >https://doi.org/10.1002/jemt.70035</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jemt.70035" target="_blank" >10.1002/jemt.70035</a>

Jazyk výsledku

angličtina

Název v původním jazyce

3D Microstructural Characterization of Human Deep Fascia Using Optical Projection Tomography, Digital Light Sheet Microscopy, and Magnetic Resonance Microscopy

Popis výsledku v původním jazyce

Traditional histological methods provide limited insights into the complex 3D microstructure of fascia and its relationship to disease. This study explored the capacity of different 3D microscopy techniques for characterizing the microstructure of fascia lata (FL) and thoracolumbar fascia (TLF). Tissues from four donors were studied using optical projection tomography (OPT), digital light sheet (DLS) microscopy, and magnetic resonance microscopy (MRM). Samples for OPT and DLS were imaged with a custom OPT scanner and the DLS arm of a Leica Stellaris microscope, respectively. MRM was performed using a 9.4 T superconducting magnet and an NMR/MRI spectrometer. Reference histological evaluation was performed to guide the interpretation of 3D data. Image analyses were performed using FIJI and Ellipse software. DLS offered superior resolution, but all techniques revealed a trilaminar structure in both fasciae: a thick, collagen-rich intermediate layer flanked by thinner layers with loose connective tissue. The FL intermediate layer was thinner (210.5–258.7 μm) with longitudinally oriented collagen, while the TLF intermediate layer was thicker (302.3–343.6 μm) with both oblique and longitudinal fibers. The superficial layer in FL was thicker (128.8–161.5 μm) than in TLF (84.65–123.10 μm) across imaging modalities. The deep layer also varied between fasciae, with 54.3–73.8 μm in FL and 44.78–70.30 μm in TLF. Layer thickness measurements did not differ significantly across techniques. This study demonstrates the feasibility of different 3D microscopy techniques for visualizing and quantifying fascia extracellular matrix structure and organization, laying the groundwork for future investigations into potential structural alterations in disease.

Název v anglickém jazyce

3D Microstructural Characterization of Human Deep Fascia Using Optical Projection Tomography, Digital Light Sheet Microscopy, and Magnetic Resonance Microscopy

Popis výsledku anglicky

Traditional histological methods provide limited insights into the complex 3D microstructure of fascia and its relationship to disease. This study explored the capacity of different 3D microscopy techniques for characterizing the microstructure of fascia lata (FL) and thoracolumbar fascia (TLF). Tissues from four donors were studied using optical projection tomography (OPT), digital light sheet (DLS) microscopy, and magnetic resonance microscopy (MRM). Samples for OPT and DLS were imaged with a custom OPT scanner and the DLS arm of a Leica Stellaris microscope, respectively. MRM was performed using a 9.4 T superconducting magnet and an NMR/MRI spectrometer. Reference histological evaluation was performed to guide the interpretation of 3D data. Image analyses were performed using FIJI and Ellipse software. DLS offered superior resolution, but all techniques revealed a trilaminar structure in both fasciae: a thick, collagen-rich intermediate layer flanked by thinner layers with loose connective tissue. The FL intermediate layer was thinner (210.5–258.7 μm) with longitudinally oriented collagen, while the TLF intermediate layer was thicker (302.3–343.6 μm) with both oblique and longitudinal fibers. The superficial layer in FL was thicker (128.8–161.5 μm) than in TLF (84.65–123.10 μm) across imaging modalities. The deep layer also varied between fasciae, with 54.3–73.8 μm in FL and 44.78–70.30 μm in TLF. Layer thickness measurements did not differ significantly across techniques. This study demonstrates the feasibility of different 3D microscopy techniques for visualizing and quantifying fascia extracellular matrix structure and organization, laying the groundwork for future investigations into potential structural alterations in disease.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30106 - Anatomy and morphology (plant science to be 1.6)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy Research and Technique

ISSN

1059-910X

e-ISSN

1097-0029

Svazek periodika

88

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

3037-3049

Kód UT WoS článku

001523672700001

EID výsledku v databázi Scopus

2-s2.0-105009884930