Zn overaccumulating Russula species clade together and use the same mechanism for the detoxification of excess Zn
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985831%3A_____%2F19%3A00504090" target="_blank" >RIV/67985831:_____/19:00504090 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60461373:22330/19:43919773
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S0045653519304989?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0045653519304989?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chemosphere.2019.03.062" target="_blank" >10.1016/j.chemosphere.2019.03.062</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Zn overaccumulating Russula species clade together and use the same mechanism for the detoxification of excess Zn
Popis výsledku v původním jazyce
It has been firmly established that macrofungi can accumulate large amounts of heavy metals in their sporocarps. However, the mechanisms of the accumulation and storage are being uncovered only recently. We have previously documented that Russula bresadolae can accumulate over 1 g Zn kg−1 dry weight and that sequestration of a substantial proportion of overaccumulated Zn involves binding with peptides, RaZBPs, seen so far only in this species. In this work we examined Zn contents of 360 sporocarp collections from unpolluted environments covering 114 species of the genus Russula. Whilst the concentrations of Zn in most analysed species were in the range of 50–150 mg kg−1, the species of subgenera Brevipes and Compactae accumulate very low Zn (< 50 mg kg−1 ). We further identified five new Zn-overaccumulating species of subgenus Russula, which form with R. bresadolae a separate phylogenetic subclade in which the sporocarp Zn concentrations ranged from 326 to 845 mg kg−1. We demonstrate that R. pumila and R. ochroleuca express at least one ZBP gene and when expressed in metal-sensitive S. cerevisiae, all ZBPs protected the yeasts against Zn (and Cd) toxicity equally well. The respective ZBPs were confirmed in the native Zn-complexes of R. pumila and R. ochroleuca, which represented 80% of Zn extracted from their sporocarps. This study is the first extensive genus-wide report of metal accumulation in macrofungi, which further demonstrates that the Zn binding with cytosolic ZBP peptides is not a trait restricted only to R. bresadolae.
Název v anglickém jazyce
Zn overaccumulating Russula species clade together and use the same mechanism for the detoxification of excess Zn
Popis výsledku anglicky
It has been firmly established that macrofungi can accumulate large amounts of heavy metals in their sporocarps. However, the mechanisms of the accumulation and storage are being uncovered only recently. We have previously documented that Russula bresadolae can accumulate over 1 g Zn kg−1 dry weight and that sequestration of a substantial proportion of overaccumulated Zn involves binding with peptides, RaZBPs, seen so far only in this species. In this work we examined Zn contents of 360 sporocarp collections from unpolluted environments covering 114 species of the genus Russula. Whilst the concentrations of Zn in most analysed species were in the range of 50–150 mg kg−1, the species of subgenera Brevipes and Compactae accumulate very low Zn (< 50 mg kg−1 ). We further identified five new Zn-overaccumulating species of subgenus Russula, which form with R. bresadolae a separate phylogenetic subclade in which the sporocarp Zn concentrations ranged from 326 to 845 mg kg−1. We demonstrate that R. pumila and R. ochroleuca express at least one ZBP gene and when expressed in metal-sensitive S. cerevisiae, all ZBPs protected the yeasts against Zn (and Cd) toxicity equally well. The respective ZBPs were confirmed in the native Zn-complexes of R. pumila and R. ochroleuca, which represented 80% of Zn extracted from their sporocarps. This study is the first extensive genus-wide report of metal accumulation in macrofungi, which further demonstrates that the Zn binding with cytosolic ZBP peptides is not a trait restricted only to R. bresadolae.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10511 - Environmental sciences (social aspects to be 5.7)
Návaznosti výsledku
Projekt
<a href="/cs/project/GA16-15065S" target="_blank" >GA16-15065S: Faktory ovlivňující akumulaci těžkých kovů velkými houbami</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Chemosphere
ISSN
0045-6535
e-ISSN
—
Svazek periodika
225
Číslo periodika v rámci svazku
June 2019
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
9
Strana od-do
618-626
Kód UT WoS článku
000467668500067
EID výsledku v databázi Scopus
2-s2.0-85062894508