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Zn overaccumulating Russula species clade together and use the same mechanism for the detoxification of excess Zn

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985831%3A_____%2F19%3A00504090" target="_blank" >RIV/67985831:_____/19:00504090 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/60461373:22330/19:43919773

  • Výsledek na webu

    <a href="https://www.sciencedirect.com/science/article/pii/S0045653519304989?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0045653519304989?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.chemosphere.2019.03.062" target="_blank" >10.1016/j.chemosphere.2019.03.062</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Zn overaccumulating Russula species clade together and use the same mechanism for the detoxification of excess Zn

  • Popis výsledku v původním jazyce

    It has been firmly established that macrofungi can accumulate large amounts of heavy metals in their sporocarps. However, the mechanisms of the accumulation and storage are being uncovered only recently. We have previously documented that Russula bresadolae can accumulate over 1 g Zn kg−1 dry weight and that sequestration of a substantial proportion of overaccumulated Zn involves binding with peptides, RaZBPs, seen so far only in this species. In this work we examined Zn contents of 360 sporocarp collections from unpolluted environments covering 114 species of the genus Russula. Whilst the concentrations of Zn in most analysed species were in the range of 50–150 mg kg−1, the species of subgenera Brevipes and Compactae accumulate very low Zn (< 50 mg kg−1 ). We further identified five new Zn-overaccumulating species of subgenus Russula, which form with R. bresadolae a separate phylogenetic subclade in which the sporocarp Zn concentrations ranged from 326 to 845 mg kg−1. We demonstrate that R. pumila and R. ochroleuca express at least one ZBP gene and when expressed in metal-sensitive S. cerevisiae, all ZBPs protected the yeasts against Zn (and Cd) toxicity equally well. The respective ZBPs were confirmed in the native Zn-complexes of R. pumila and R. ochroleuca, which represented 80% of Zn extracted from their sporocarps. This study is the first extensive genus-wide report of metal accumulation in macrofungi, which further demonstrates that the Zn binding with cytosolic ZBP peptides is not a trait restricted only to R. bresadolae.

  • Název v anglickém jazyce

    Zn overaccumulating Russula species clade together and use the same mechanism for the detoxification of excess Zn

  • Popis výsledku anglicky

    It has been firmly established that macrofungi can accumulate large amounts of heavy metals in their sporocarps. However, the mechanisms of the accumulation and storage are being uncovered only recently. We have previously documented that Russula bresadolae can accumulate over 1 g Zn kg−1 dry weight and that sequestration of a substantial proportion of overaccumulated Zn involves binding with peptides, RaZBPs, seen so far only in this species. In this work we examined Zn contents of 360 sporocarp collections from unpolluted environments covering 114 species of the genus Russula. Whilst the concentrations of Zn in most analysed species were in the range of 50–150 mg kg−1, the species of subgenera Brevipes and Compactae accumulate very low Zn (< 50 mg kg−1 ). We further identified five new Zn-overaccumulating species of subgenus Russula, which form with R. bresadolae a separate phylogenetic subclade in which the sporocarp Zn concentrations ranged from 326 to 845 mg kg−1. We demonstrate that R. pumila and R. ochroleuca express at least one ZBP gene and when expressed in metal-sensitive S. cerevisiae, all ZBPs protected the yeasts against Zn (and Cd) toxicity equally well. The respective ZBPs were confirmed in the native Zn-complexes of R. pumila and R. ochroleuca, which represented 80% of Zn extracted from their sporocarps. This study is the first extensive genus-wide report of metal accumulation in macrofungi, which further demonstrates that the Zn binding with cytosolic ZBP peptides is not a trait restricted only to R. bresadolae.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    10511 - Environmental sciences (social aspects to be 5.7)

Návaznosti výsledku

  • Projekt

    <a href="/cs/project/GA16-15065S" target="_blank" >GA16-15065S: Faktory ovlivňující akumulaci těžkých kovů velkými houbami</a><br>

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2019

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Chemosphere

  • ISSN

    0045-6535

  • e-ISSN

  • Svazek periodika

    225

  • Číslo periodika v rámci svazku

    June 2019

  • Stát vydavatele periodika

    GB - Spojené království Velké Británie a Severního Irska

  • Počet stran výsledku

    9

  • Strana od-do

    618-626

  • Kód UT WoS článku

    000467668500067

  • EID výsledku v databázi Scopus

    2-s2.0-85062894508