The vacuum cast embedding and X-ray micro-computed tomography as standard methods to visualise bioerosion traces in Serpulid tubes

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The vacuum cast embedding and X-ray micro-computed tomography as standard methods to visualise bioerosion traces in Serpulid tubes

Popis výsledku v původním jazyce

Bioerosion in tubes of serpulid polychaetes from the late Cenomanian and early Turonian of the Bohemian Cretaceous Basin in the Czech Republic and the late Cenomanian of Le Mans region in France were studied by combination of non-invasive micro-computed tomography and invasive vacuum cast-embedding technique producing polymer resin casts. Our study shows how different imaging techniques bias the description and diagnosis of bioerosion inside tiny serpulid tubes (0.5–0.8 mm in diameter). The great benefits of micro-CT are its non-destructive nature and ability to display empty borings (air space) or borings filled with dense matrix. The results, however, depend critically on the contrast in density and atomic number between the fossil material and the respective boring infilling. Moreover, resolutions of less than 5 μm cannot be effectively achieved. Vacuum cast-embedding is the process of filling tunnels with epoxy resin under vacuum conditions, and dissolving the substrate via treatment with diluted hydrochloric acid. This method is thus unsuitable for poorly soluble (e.g. silicified, pyritized) substrates, boreholes filled with non-porous material, and due to its destructive nature for unique or rare specimens. Nevertheless, the SEM images of the resulting casts have significantly better resolution than micro-CT and thus enable to display and quantify the most delicate morphological features such as microborings as well as details of macroborings.n

Název v anglickém jazyce

The vacuum cast embedding and X-ray micro-computed tomography as standard methods to visualise bioerosion traces in Serpulid tubes

Popis výsledku anglicky

Bioerosion in tubes of serpulid polychaetes from the late Cenomanian and early Turonian of the Bohemian Cretaceous Basin in the Czech Republic and the late Cenomanian of Le Mans region in France were studied by combination of non-invasive micro-computed tomography and invasive vacuum cast-embedding technique producing polymer resin casts. Our study shows how different imaging techniques bias the description and diagnosis of bioerosion inside tiny serpulid tubes (0.5–0.8 mm in diameter). The great benefits of micro-CT are its non-destructive nature and ability to display empty borings (air space) or borings filled with dense matrix. The results, however, depend critically on the contrast in density and atomic number between the fossil material and the respective boring infilling. Moreover, resolutions of less than 5 μm cannot be effectively achieved. Vacuum cast-embedding is the process of filling tunnels with epoxy resin under vacuum conditions, and dissolving the substrate via treatment with diluted hydrochloric acid. This method is thus unsuitable for poorly soluble (e.g. silicified, pyritized) substrates, boreholes filled with non-porous material, and due to its destructive nature for unique or rare specimens. Nevertheless, the SEM images of the resulting casts have significantly better resolution than micro-CT and thus enable to display and quantify the most delicate morphological features such as microborings as well as details of macroborings.n

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10506 - Paleontology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů