Vliv naftalenových a salicylátových anoalogů na bioluminiscenci bioreportéru Pseudomonas fluorescens HK44.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F07%3A00081958" target="_blank" >RIV/67985858:_____/07:00081958 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of Naphthalene and Salicylate Analogues on the Bioluminescence of Bioreporter Pseudomonas Fluorescens HK44.

Popis výsledku v původním jazyce

Pseudomonas fluorescens HK44 is a bioluminescence bioreporter harbouring the nah-lux construct. The bacterium is capable of selective luminescence in the presence of naphthalene and/or intermidiate of its metabolism salicylate. The induction proceeds viabinding of salicylate to the nahR gene product which results in increase of expression of nah and lux genes. In this work, 74 compounds were tested for the induction of bioluminescence of P. fluorescens HK44. Among these compounds there were substitutednaphthalenes, naphthalene analogues (e.g. azulene), substituted salicylic acids, salicylic acids analogues (e.g. 2-aminobenzoic acid), polyaromatic hydrocarbons and intermediates of naphthalene metabolism. Results show, that many compounds induce at least slightly bioluminescence of P. fluorescens HK44. Few compounds were found to be even better inducers than naphthalene. Higher concentrations of majority of compounds have on the other hand inhibitory effect on the bioluminescence.

Název v anglickém jazyce

Effect of Naphthalene and Salicylate Analogues on the Bioluminescence of Bioreporter Pseudomonas Fluorescens HK44.

Popis výsledku anglicky

Pseudomonas fluorescens HK44 is a bioluminescence bioreporter harbouring the nah-lux construct. The bacterium is capable of selective luminescence in the presence of naphthalene and/or intermidiate of its metabolism salicylate. The induction proceeds viabinding of salicylate to the nahR gene product which results in increase of expression of nah and lux genes. In this work, 74 compounds were tested for the induction of bioluminescence of P. fluorescens HK44. Among these compounds there were substitutednaphthalenes, naphthalene analogues (e.g. azulene), substituted salicylic acids, salicylic acids analogues (e.g. 2-aminobenzoic acid), polyaromatic hydrocarbons and intermediates of naphthalene metabolism. Results show, that many compounds induce at least slightly bioluminescence of P. fluorescens HK44. Few compounds were found to be even better inducers than naphthalene. Higher concentrations of majority of compounds have on the other hand inhibitory effect on the bioluminescence.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Svazek periodika

52

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

3-14

Kód UT WoS článku

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EID výsledku v databázi Scopus

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