Whole-Cell Optical Biosensor for Mercury ? Operational Conditions in Saline Water

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F15%3A00443171" target="_blank" >RIV/67985858:_____/15:00443171 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1515/chempap-2015-0009" target="_blank" >http://dx.doi.org/10.1515/chempap-2015-0009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/chempap-2015-0009" target="_blank" >10.1515/chempap-2015-0009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Whole-Cell Optical Biosensor for Mercury ? Operational Conditions in Saline Water

Popis výsledku v původním jazyce

The present study demonstrates the influences of chlorides, fluorides and bromides of potassium and sodium on the growth and Hg2+-induced bioluminescence of bioreporter Escherichia coli ARL1. In a Luria-Bertani medium,cell growth was inhibited by concentrations of sodium and potassium fluorides above 0.2 mol L-1. The addition of NaCl increased cell tolerance to the toxic effects of fluorides and bromides. Lag periods of 10 h and more were observed for cultivations in LB without NaCl and with halides atconcentrations lower than 0.06 mol L-1. In a phosphate buffer (PB), the bioluminescence of E. coli ARL1, induced with HgCl2, was increased by the addition of NaCl, KCl, NaBr, KBr, NaF and KF (concentration of 0-0.25 mol L-1). In a saline phosphate buffer(PBS), the maxima of induced bioluminescence declined to 50%, in the case of NaF (0.12 mol L-1), and to zero for KF. An addition of tryptone to the induction medium increased induced light emission ten-fold. Concentrated artificial sea w

Název v anglickém jazyce

Whole-Cell Optical Biosensor for Mercury ? Operational Conditions in Saline Water

Popis výsledku anglicky

The present study demonstrates the influences of chlorides, fluorides and bromides of potassium and sodium on the growth and Hg2+-induced bioluminescence of bioreporter Escherichia coli ARL1. In a Luria-Bertani medium,cell growth was inhibited by concentrations of sodium and potassium fluorides above 0.2 mol L-1. The addition of NaCl increased cell tolerance to the toxic effects of fluorides and bromides. Lag periods of 10 h and more were observed for cultivations in LB without NaCl and with halides atconcentrations lower than 0.06 mol L-1. In a phosphate buffer (PB), the bioluminescence of E. coli ARL1, induced with HgCl2, was increased by the addition of NaCl, KCl, NaBr, KBr, NaF and KF (concentration of 0-0.25 mol L-1). In a saline phosphate buffer(PBS), the maxima of induced bioluminescence declined to 50%, in the case of NaF (0.12 mol L-1), and to zero for KF. An addition of tryptone to the induction medium increased induced light emission ten-fold. Concentrated artificial sea w

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CA - Anorganická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/TA03010544" target="_blank" >TA03010544: Tištěné optické chemické senzory (TOS).</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemical Papers

ISSN

0366-6352

e-ISSN

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Svazek periodika

69

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

9

Strana od-do

183-191

Kód UT WoS článku

000350130100017

EID výsledku v databázi Scopus

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