Label-Free Biosensing in Complex Media: A Referencing Approach

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F13%3A00396516" target="_blank" >RIV/67985882:_____/13:00396516 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1021/ac401062m" target="_blank" >http://dx.doi.org/10.1021/ac401062m</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/ac401062m" target="_blank" >10.1021/ac401062m</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Label-Free Biosensing in Complex Media: A Referencing Approach

Popis výsledku v původním jazyce

We present a novel approach to reference-compensated label-free affinity biosensing in complex media. Unlike conventional approaches that employ surfaces with different biological functionalities in the detection and reference channels to produce a reference-compensated sensor response, the new approach (referred as to single surface referencing (SSR)) uses a single functionalized surface split into the detection and reference channel to which complex sample (detection channel) and complex sample mixedwith biomolecules binding to the analyte and thus inhibiting the binding of the analyte to the functionalized surface (reference channel) is introduced. This approach ensures that (i) only the detection channel captures the analyte and (ii) nonspecific binding incurred in the detection and reference channels are the same. We evaluate this approach in a model biosensing experiment, detection of a cancer biomarker carcinoembryonic antigen (CEA) in blood plasma using antibody against CEA an

Název v anglickém jazyce

Label-Free Biosensing in Complex Media: A Referencing Approach

Popis výsledku anglicky

We present a novel approach to reference-compensated label-free affinity biosensing in complex media. Unlike conventional approaches that employ surfaces with different biological functionalities in the detection and reference channels to produce a reference-compensated sensor response, the new approach (referred as to single surface referencing (SSR)) uses a single functionalized surface split into the detection and reference channel to which complex sample (detection channel) and complex sample mixedwith biomolecules binding to the analyte and thus inhibiting the binding of the analyte to the functionalized surface (reference channel) is introduced. This approach ensures that (i) only the detection channel captures the analyte and (ii) nonspecific binding incurred in the detection and reference channels are the same. We evaluate this approach in a model biosensing experiment, detection of a cancer biomarker carcinoembryonic antigen (CEA) in blood plasma using antibody against CEA an

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JB - Senzory, čidla, měření a regulace

OECD FORD obor

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Projekt

<a href="/cs/project/LH11102" target="_blank" >LH11102: Nanoplasmonické materiály vytvářené pomocí samoorganizačních procesů a jejich využití v senzorech</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Chemistry

ISSN

0003-2700

e-ISSN

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Svazek periodika

85

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

5637-5640

Kód UT WoS článku

000320749200005

EID výsledku v databázi Scopus

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