Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F16%3A00469628" target="_blank" >RIV/67985882:_____/16:00469628 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/16:10327964 RIV/00064165:_____/16:10327964

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00216-016-9664-z" target="_blank" >http://dx.doi.org/10.1007/s00216-016-9664-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-016-9664-z" target="_blank" >10.1007/s00216-016-9664-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples

Popis výsledku v původním jazyce

Pregnancy associated plasma protein A2 (PAPP-A2) is a metalloproteinase that plays multiple roles in fetal development and post-natal growth. Here we present a novel surface plasmon resonance (SPR) biosensor for the rapid and quantitative detection of PAPP-A2 in blood samples. This biosensor uses a single surface referencing approach and a sandwich assay with functionalized gold nanoparticles for signal enhancement. We demonstrate that this SPR biosensor enables the detection of PAPP-A2 in 30 % blood plasma at levels as low as 3.6 ng/mL. We also characterize the performance of the biosensor and evaluate its cross-reactivity to a PAPP-A analogue. Finally, we utilize this SPR biosensor for the detection of PAPP-A2 in blood serum from two groups of subjects: pregnant women and healthy non-pregnant women and men

Název v anglickém jazyce

Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples

Popis výsledku anglicky

Pregnancy associated plasma protein A2 (PAPP-A2) is a metalloproteinase that plays multiple roles in fetal development and post-natal growth. Here we present a novel surface plasmon resonance (SPR) biosensor for the rapid and quantitative detection of PAPP-A2 in blood samples. This biosensor uses a single surface referencing approach and a sandwich assay with functionalized gold nanoparticles for signal enhancement. We demonstrate that this SPR biosensor enables the detection of PAPP-A2 in 30 % blood plasma at levels as low as 3.6 ng/mL. We also characterize the performance of the biosensor and evaluate its cross-reactivity to a PAPP-A analogue. Finally, we utilize this SPR biosensor for the detection of PAPP-A2 in blood serum from two groups of subjects: pregnant women and healthy non-pregnant women and men

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

—

Projekt

<a href="/cs/project/GBP205%2F12%2FG118" target="_blank" >GBP205/12/G118: Nanobiofotonika pro medicínu budoucnosti</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

—

Svazek periodika

408

Číslo periodika v rámci svazku

26

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

7265-7269

Kód UT WoS článku

000385155200007

EID výsledku v databázi Scopus

2-s2.0-84974824534