Surface plasmon resonance biosensor for the detection of tau-amyloid beta complex

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F20%3A00537413" target="_blank" >RIV/67985882:_____/20:00537413 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/20:00072902

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.snb.2020.128146" target="_blank" >http://dx.doi.org/10.1016/j.snb.2020.128146</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2020.128146" target="_blank" >10.1016/j.snb.2020.128146</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Surface plasmon resonance biosensor for the detection of tau-amyloid beta complex

Popis výsledku v původním jazyce

The development of methods advancing the understanding of molecular processes related to Alzheimer's disease (AD) and detection of biomarkers of AD presents an important research goal. We present a new biosensor-based method for the detection of a prospective biomarker of AD, the complex of protein tau and amyloid beta (tau-A beta). The detection method combines a new sandwich assay incorporating functionalized gold nanoparticles (AuNPs) with a surface plasmon resonance (SPR) biosensor. We optimize the assay with respect to the functionalization of the sensor surface and the choice of AuNPs and show that the sensor allows for the detection of the tau-A beta complex in cerebrospinal fluid (CSF) with the limit of detection of 1 pM. We also present the results of a preliminary study in which we use the method to detect tau-A beta complex in CSF samples

Název v anglickém jazyce

Surface plasmon resonance biosensor for the detection of tau-amyloid beta complex

Popis výsledku anglicky

The development of methods advancing the understanding of molecular processes related to Alzheimer's disease (AD) and detection of biomarkers of AD presents an important research goal. We present a new biosensor-based method for the detection of a prospective biomarker of AD, the complex of protein tau and amyloid beta (tau-A beta). The detection method combines a new sandwich assay incorporating functionalized gold nanoparticles (AuNPs) with a surface plasmon resonance (SPR) biosensor. We optimize the assay with respect to the functionalization of the sensor surface and the choice of AuNPs and show that the sensor allows for the detection of the tau-A beta complex in cerebrospinal fluid (CSF) with the limit of detection of 1 pM. We also present the results of a preliminary study in which we use the method to detect tau-A beta complex in CSF samples

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

<a href="/cs/project/NV16-27611A" target="_blank" >NV16-27611A: Interakce intracelulárního amyloidu beta a diagnostika Alzheimerovy nemoci</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors and Actuators B - Chemical

ISSN

0925-4005

e-ISSN

—

Svazek periodika

316

Číslo periodika v rámci svazku

15 JUL

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

4

Strana od-do

128146

Kód UT WoS článku

000533550100001

EID výsledku v databázi Scopus

2-s2.0-85084243290