Ionic Environment Affects Biomolecular Interactions of Amyloid-beta: SPR Biosensor Study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F20%3A00537414" target="_blank" >RIV/67985882:_____/20:00537414 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.3390/ijms21249727" target="_blank" >https://doi.org/10.3390/ijms21249727</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms21249727" target="_blank" >10.3390/ijms21249727</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ionic Environment Affects Biomolecular Interactions of Amyloid-beta: SPR Biosensor Study

Popis výsledku v původním jazyce

In early stages of Alzheimer's disease (AD), amyloid beta (A beta) accumulates in the mitochondrial matrix and interacts with mitochondrial proteins, such as cyclophilin D (cypD) and 17 beta-hydroxysteroid dehydrogenase 10 (17 beta-HSD10). Multiple processes associated with AD such as increased production or oligomerization of A beta affect these interactions and disbalance the equilibrium between the biomolecules, which contributes to mitochondrial dysfunction. Here, we investigate the effect of the ionic environment on the interactions of A beta (A beta(1-40), A beta(1-42)) with cypD and 17 beta-HSD10 using a surface plasmon resonance (SPR) biosensor. We show that changes in concentrations of K+ and Mg2+ significantly affect the interactions and may increase the binding efficiency between the biomolecules by up to 35% and 65% for the interactions with A beta(1-40) and A beta(1-42), respectively, in comparison with the physiological state. We also demonstrate that while the binding of A beta(1-40) to cypD and 17 beta-HSD10 takes place preferentially around the physiological concentrations of ions, decreased concentrations of K+ and increased concentrations of Mg2+ promote the interaction of both mitochondrial proteins with A beta(1-42). These results suggest that the ionic environment represents an important factor that should be considered in the investigation of biomolecular interactions taking place in the mitochondrial matrix under physiological as well as AD-associated conditions

Název v anglickém jazyce

Ionic Environment Affects Biomolecular Interactions of Amyloid-beta: SPR Biosensor Study

Popis výsledku anglicky

In early stages of Alzheimer's disease (AD), amyloid beta (A beta) accumulates in the mitochondrial matrix and interacts with mitochondrial proteins, such as cyclophilin D (cypD) and 17 beta-hydroxysteroid dehydrogenase 10 (17 beta-HSD10). Multiple processes associated with AD such as increased production or oligomerization of A beta affect these interactions and disbalance the equilibrium between the biomolecules, which contributes to mitochondrial dysfunction. Here, we investigate the effect of the ionic environment on the interactions of A beta (A beta(1-40), A beta(1-42)) with cypD and 17 beta-HSD10 using a surface plasmon resonance (SPR) biosensor. We show that changes in concentrations of K+ and Mg2+ significantly affect the interactions and may increase the binding efficiency between the biomolecules by up to 35% and 65% for the interactions with A beta(1-40) and A beta(1-42), respectively, in comparison with the physiological state. We also demonstrate that while the binding of A beta(1-40) to cypD and 17 beta-HSD10 takes place preferentially around the physiological concentrations of ions, decreased concentrations of K+ and increased concentrations of Mg2+ promote the interaction of both mitochondrial proteins with A beta(1-42). These results suggest that the ionic environment represents an important factor that should be considered in the investigation of biomolecular interactions taking place in the mitochondrial matrix under physiological as well as AD-associated conditions

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

24

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

9727

Kód UT WoS článku

000602991100001

EID výsledku v databázi Scopus

2-s2.0-85098152669