Interaction of Tris with DNA molecules and carboxylic groups on self-assembled monolayers of alkanethiols measured with surface plasmon resonance

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F21%3A00543285" target="_blank" >RIV/67985882:_____/21:00543285 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.apsusc.2021.148984" target="_blank" >https://doi.org/10.1016/j.apsusc.2021.148984</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.apsusc.2021.148984" target="_blank" >10.1016/j.apsusc.2021.148984</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of Tris with DNA molecules and carboxylic groups on self-assembled monolayers of alkanethiols measured with surface plasmon resonance

Popis výsledku v původním jazyce

Functional materials employing organic coatings on inorganic substrates are perceived as potential platforms for applications in a variety of fields. Therefore, the investigation of interactions of such systems with the micro-environment has become an important research direction in surface science. Herein, we study the interaction of one of the buffers most commonly used in biological studies, Tris buffer, with self-assembled monolayers (SAMs) of alkanethiols and short DNAs using a surface plasmon resonance (SPR) biosensor. We show that the interaction between Tris and carboxylic groups of SAMs is a complex multiphasic process. We demonstrate that Tris base binds to the protonated carboxylic groups. When those groups become deprotonated, Tris base dissociates and Tris acid is attracted, which results in the formation of a diffuse layer over the charged surface. In addition, we show that the interaction of Tris with the immobilized DNA molecules biases the determination of surface concentrations of the immobilized DNA molecules and thus also the determination of hybridization efficiencies

Název v anglickém jazyce

Interaction of Tris with DNA molecules and carboxylic groups on self-assembled monolayers of alkanethiols measured with surface plasmon resonance

Popis výsledku anglicky

Functional materials employing organic coatings on inorganic substrates are perceived as potential platforms for applications in a variety of fields. Therefore, the investigation of interactions of such systems with the micro-environment has become an important research direction in surface science. Herein, we study the interaction of one of the buffers most commonly used in biological studies, Tris buffer, with self-assembled monolayers (SAMs) of alkanethiols and short DNAs using a surface plasmon resonance (SPR) biosensor. We show that the interaction between Tris and carboxylic groups of SAMs is a complex multiphasic process. We demonstrate that Tris base binds to the protonated carboxylic groups. When those groups become deprotonated, Tris base dissociates and Tris acid is attracted, which results in the formation of a diffuse layer over the charged surface. In addition, we show that the interaction of Tris with the immobilized DNA molecules biases the determination of surface concentrations of the immobilized DNA molecules and thus also the determination of hybridization efficiencies

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10301 - Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)

Projekt

<a href="/cs/project/GA19-02739S" target="_blank" >GA19-02739S: Plasmonické biosenzory pro studium biomolekulárních interakcí</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Surface Science

ISSN

0169-4332

e-ISSN

1873-5584

Svazek periodika

546

Číslo periodika v rámci svazku

APR

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

148984

Kód UT WoS článku

000620786300005

EID výsledku v databázi Scopus

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