Ex-Vivo Measurement of the pH in Aqueous Humor Samples by a Tapered Fiber-Optic Sensor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F21%3A00553147" target="_blank" >RIV/67985882:_____/21:00553147 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/21:00351178

Výsledek na webu

<a href="https://doi.org/10.3390/s21155075" target="_blank" >https://doi.org/10.3390/s21155075</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/s21155075" target="_blank" >10.3390/s21155075</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ex-Vivo Measurement of the pH in Aqueous Humor Samples by a Tapered Fiber-Optic Sensor

Popis výsledku v původním jazyce

A practical demonstration of pH measurement in real biological samples with an in-house developed fiber-optic pH sensor system is presented. The sensor uses 8-hydroxypyrene-1,3,6-trisulfonate (HPTS) fluorescent dye as the opto-chemical transducer. The dye is immobilized in a hybrid sol-gel matrix at the tip of a tapered optical fiber. We used 405 nm and 450 nm laser diodes for the dye excitation and a photomultiplier tube as a detector. The sensor was used for the measurement of pH in human aqueous humor samples during cataract surgery. Two groups of patients were tested, one underwent conventional phacoemulsification removal of the lens while the other was subjected to femtosecond laser assisted cataract surgery (FLACS). The precision of the measurement was +/- 0.04 pH units. The average pH of the aqueous humor of patients subjected to FLACS and those subjected to phacoemulsification were 7.24 +/- 0.17 and 7.31 +/- 0.20 respectively

Název v anglickém jazyce

Ex-Vivo Measurement of the pH in Aqueous Humor Samples by a Tapered Fiber-Optic Sensor

Popis výsledku anglicky

A practical demonstration of pH measurement in real biological samples with an in-house developed fiber-optic pH sensor system is presented. The sensor uses 8-hydroxypyrene-1,3,6-trisulfonate (HPTS) fluorescent dye as the opto-chemical transducer. The dye is immobilized in a hybrid sol-gel matrix at the tip of a tapered optical fiber. We used 405 nm and 450 nm laser diodes for the dye excitation and a photomultiplier tube as a detector. The sensor was used for the measurement of pH in human aqueous humor samples during cataract surgery. Two groups of patients were tested, one underwent conventional phacoemulsification removal of the lens while the other was subjected to femtosecond laser assisted cataract surgery (FLACS). The precision of the measurement was +/- 0.04 pH units. The average pH of the aqueous humor of patients subjected to FLACS and those subjected to phacoemulsification were 7.24 +/- 0.17 and 7.31 +/- 0.20 respectively

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20201 - Electrical and electronic engineering

Projekt

<a href="/cs/project/TA04011400" target="_blank" >TA04011400: Vláknově optický pH metr pro mikroskopické biologické vzorky</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors

ISSN

1424-8220

e-ISSN

1424-8220

Svazek periodika

21

Číslo periodika v rámci svazku

15

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

10

Strana od-do

5075

Kód UT WoS článku

000682308400001

EID výsledku v databázi Scopus

2-s2.0-85111153426