The Influence of Purification of Carp Collagen Used in a Novel Composite Graft with Sandwich Construction of the Wall on Its Biological Properties and Graft Patency Rates

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985891%3A_____%2F19%3A00511739" target="_blank" >RIV/67985891:_____/19:00511739 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/19:10398593 RIV/68407700:21220/19:00336906 RIV/00023001:_____/19:00078319 RIV/00064165:_____/19:10398593

Výsledek na webu

<a href="https://www.biomed.cas.cz/physiolres/pdf/2019/68_603.pdf" target="_blank" >https://www.biomed.cas.cz/physiolres/pdf/2019/68_603.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.33549/physiolres.934117" target="_blank" >10.33549/physiolres.934117</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Influence of Purification of Carp Collagen Used in a Novel Composite Graft with Sandwich Construction of the Wall on Its Biological Properties and Graft Patency Rates

Popis výsledku v původním jazyce

We compared graft outcome between two types of a novel composite three-layer carp-collagen-coated vascular graft in low-flow conditions in a sheep model. Collagen in group A underwent more cycles of purification than in group B in order to increase the ratio between collagen and residual fat. The grafts were implanted end-to-side in both carotid arteries in sheep (14 grafts in 7 sheep in group A, 18 grafts in 9 sheep in group B) and artificially stenosed on the right side. The flow in the grafts in group A decreased from 297 +/- 118 ml/min to 158 +/- 159 ml/min (p=0.041) after placement of the artificial stenosis in group A, and from 330 +/- 164ml/min to 97 +/- 29 ml/min (p=0.0052) in group B (p=0.27 between the groups). From the five surviving animals in group A, both grafts occluded in one animal 3 and 14 days after implantation. In group B, from the six surviving animals, only one graft on the left side remained patent (p=0.0017). Histology showed degradation of the intimal layer in the center with endothelization from the periphery in group A and formation of thick fibrous intimal layer in group B. We conclude that the ratio between collagen and lipid content in the novel three-layer graft plays a critical role in its patency and structural changes in vivo.

Název v anglickém jazyce

The Influence of Purification of Carp Collagen Used in a Novel Composite Graft with Sandwich Construction of the Wall on Its Biological Properties and Graft Patency Rates

Popis výsledku anglicky

We compared graft outcome between two types of a novel composite three-layer carp-collagen-coated vascular graft in low-flow conditions in a sheep model. Collagen in group A underwent more cycles of purification than in group B in order to increase the ratio between collagen and residual fat. The grafts were implanted end-to-side in both carotid arteries in sheep (14 grafts in 7 sheep in group A, 18 grafts in 9 sheep in group B) and artificially stenosed on the right side. The flow in the grafts in group A decreased from 297 +/- 118 ml/min to 158 +/- 159 ml/min (p=0.041) after placement of the artificial stenosis in group A, and from 330 +/- 164ml/min to 97 +/- 29 ml/min (p=0.0052) in group B (p=0.27 between the groups). From the five surviving animals in group A, both grafts occluded in one animal 3 and 14 days after implantation. In group B, from the six surviving animals, only one graft on the left side remained patent (p=0.0017). Histology showed degradation of the intimal layer in the center with endothelization from the periphery in group A and formation of thick fibrous intimal layer in group B. We conclude that the ratio between collagen and lipid content in the novel three-layer graft plays a critical role in its patency and structural changes in vivo.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20601 - Medical engineering

Projekt

<a href="/cs/project/NV15-27941A" target="_blank" >NV15-27941A: Využití neantigenního rybího kolagenu při konstrukci implantátů a jako nosiče léků</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

—

Svazek periodika

68

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

603-610

Kód UT WoS článku

000483374200008

EID výsledku v databázi Scopus

2-s2.0-85071715123