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Peptide mass mapping in bioapatites isolated from animal bones

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985891%3A_____%2F20%3A00531743" target="_blank" >RIV/67985891:_____/20:00531743 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/60461373:22330/20:43921498

  • Výsledek na webu

    <a href="https://link.springer.com/article/10.1007/s10856-020-06371-z" target="_blank" >https://link.springer.com/article/10.1007/s10856-020-06371-z</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s10856-020-06371-z" target="_blank" >10.1007/s10856-020-06371-z</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Peptide mass mapping in bioapatites isolated from animal bones

  • Popis výsledku v původním jazyce

    Bioapatite ceramics produced from biogenic sources provide highly attractive materials for the preparation of artificial replacements since such materials are not only more easily accepted by living organisms, but bioapatite isolated from biowaste such as xenogeneous bones also provides a low-cost material. Nevertheless, the presence of organic compounds in the bioapatite may lead to a deterioration in its quality and may trigger an undesirable immune response. Therefore, procedures which ensure the elimination of organic compounds through bioapatite isolation are being subjected to intense investigation and the presence of remaining organic impurities is being determined through the application of various methods. Since current conclusions concerning the conditions suitable for the elimination of organic compounds remain ambiguous, we used the mass spectrometry-based proteomic approach in order to determine the presence of proteins or peptides in bioapatite samples treated under the most frequently employed conditions, i.e., the alkaline hydrothermal proces and calcination at 500 °C. Since we also investigated the presence of proteins or peptides in treated bioapatite particles of differing sizes, we discovered that both calcination and the size of the bioapatite particles constitute the main factors influencing the presence of proteins or peptides in bioapatite. In fact, while intact proteins were detected even in calcinated bioapatite consisting of particles >250 μm, no proteins were detected in the same material consisting of particles <40 μm. Therefore, we recommend the use of powdered bioapatite for the preparation of artificial replacements since it is, more effectively purified than apatite in the form of blocks. In addition, we observed that while alkaline hydrothermal treatment leads to the non-specific cleavage of proteins, it does not ensure the full degradation thereof.

  • Název v anglickém jazyce

    Peptide mass mapping in bioapatites isolated from animal bones

  • Popis výsledku anglicky

    Bioapatite ceramics produced from biogenic sources provide highly attractive materials for the preparation of artificial replacements since such materials are not only more easily accepted by living organisms, but bioapatite isolated from biowaste such as xenogeneous bones also provides a low-cost material. Nevertheless, the presence of organic compounds in the bioapatite may lead to a deterioration in its quality and may trigger an undesirable immune response. Therefore, procedures which ensure the elimination of organic compounds through bioapatite isolation are being subjected to intense investigation and the presence of remaining organic impurities is being determined through the application of various methods. Since current conclusions concerning the conditions suitable for the elimination of organic compounds remain ambiguous, we used the mass spectrometry-based proteomic approach in order to determine the presence of proteins or peptides in bioapatite samples treated under the most frequently employed conditions, i.e., the alkaline hydrothermal proces and calcination at 500 °C. Since we also investigated the presence of proteins or peptides in treated bioapatite particles of differing sizes, we discovered that both calcination and the size of the bioapatite particles constitute the main factors influencing the presence of proteins or peptides in bioapatite. In fact, while intact proteins were detected even in calcinated bioapatite consisting of particles >250 μm, no proteins were detected in the same material consisting of particles <40 μm. Therefore, we recommend the use of powdered bioapatite for the preparation of artificial replacements since it is, more effectively purified than apatite in the form of blocks. In addition, we observed that while alkaline hydrothermal treatment leads to the non-specific cleavage of proteins, it does not ensure the full degradation thereof.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    20601 - Medical engineering

Návaznosti výsledku

  • Projekt

    <a href="/cs/project/NV15-25813A" target="_blank" >NV15-25813A: Vývoj a komplexní preklinické testování nových kompozitních materiálů pro kostní chirurgii</a><br>

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2020

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Journal of Materials Science-Materials in Medicine

  • ISSN

    0957-4530

  • e-ISSN

  • Svazek periodika

    31

  • Číslo periodika v rámci svazku

    3

  • Stát vydavatele periodika

    NL - Nizozemsko

  • Počet stran výsledku

    11

  • Strana od-do

    32

  • Kód UT WoS článku

    000521322700001

  • EID výsledku v databázi Scopus

    2-s2.0-85081173067