Microcomputed tomographic, biomechanical and histological analyses of lumbar interbody fusion with iliac crest bone graft in a pig model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985891%3A_____%2F21%3A00542711" target="_blank" >RIV/67985891:_____/21:00542711 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21220/21:00357107 RIV/65269705:_____/21:00074474 RIV/00216305:26620/21:PU142047 RIV/00216224:14110/21:00122157

Výsledek na webu

<a href="https://actavet.vfu.cz/90/1/0069/" target="_blank" >https://actavet.vfu.cz/90/1/0069/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202190010069" target="_blank" >10.2754/avb202190010069</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Microcomputed tomographic, biomechanical and histological analyses of lumbar interbody fusion with iliac crest bone graft in a pig model

Popis výsledku v původním jazyce

The main goal of this study was to assess the progress of vertebral stability after lumbar interbody fusion related to microcomputed tomography (micro CT), biomechanical analysis, and histological assessment towards spine fusion. Twelve male pigs were used, each underwent L2-3 discectomy and implantation of an iliac crest bone graft in two groups, six spines were harvested eight weeks (A1) and six spines 16 weeks (A2) after surgery (7 native spines for biomechanical analysis). The CT was performed by GE phoenix datos|x 2.0 with a sample drift correction. The samples were divided according to fusion quality. Biomechanical evaluation was carried out on the MTS Mini Bionix testing system. In the nondestructive mode, three cycles of pure bending moments were applied (5 Nm load limit) at a rate of 20 °/min in flexion (+40 °) and extension (-40 °). Two representative histological sections from four samples were obtained (A1, n = 2, A2, n = 2), areas of mature bone were quantified. In micro CT, better results were achieved in group A2 (not significant). Eight weeks after the operation, flexural stiffness decreased to 48% of its initial value for native cadavers (P < 0.05), after 16 weeks it was comparable to native cadavers, demonstrating the suitability of the implanted graft (P < 0.05). The newly formed bone tissue occupied an average area of 94.205 mm (A1) and 26.240 mm (A2). It was confirmed that micro CT, biomechanical analysis, and histological assessment are technically feasible and suitable for the evaluation of results of other methods of large bone defect treatment. 2 2

Název v anglickém jazyce

Microcomputed tomographic, biomechanical and histological analyses of lumbar interbody fusion with iliac crest bone graft in a pig model

Popis výsledku anglicky

The main goal of this study was to assess the progress of vertebral stability after lumbar interbody fusion related to microcomputed tomography (micro CT), biomechanical analysis, and histological assessment towards spine fusion. Twelve male pigs were used, each underwent L2-3 discectomy and implantation of an iliac crest bone graft in two groups, six spines were harvested eight weeks (A1) and six spines 16 weeks (A2) after surgery (7 native spines for biomechanical analysis). The CT was performed by GE phoenix datos|x 2.0 with a sample drift correction. The samples were divided according to fusion quality. Biomechanical evaluation was carried out on the MTS Mini Bionix testing system. In the nondestructive mode, three cycles of pure bending moments were applied (5 Nm load limit) at a rate of 20 °/min in flexion (+40 °) and extension (-40 °). Two representative histological sections from four samples were obtained (A1, n = 2, A2, n = 2), areas of mature bone were quantified. In micro CT, better results were achieved in group A2 (not significant). Eight weeks after the operation, flexural stiffness decreased to 48% of its initial value for native cadavers (P < 0.05), after 16 weeks it was comparable to native cadavers, demonstrating the suitability of the implanted graft (P < 0.05). The newly formed bone tissue occupied an average area of 94.205 mm (A1) and 26.240 mm (A2). It was confirmed that micro CT, biomechanical analysis, and histological assessment are technically feasible and suitable for the evaluation of results of other methods of large bone defect treatment. 2 2

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20601 - Medical engineering

Projekt

<a href="/cs/project/NV17-31276A" target="_blank" >NV17-31276A: Všestranné preklinické zhodnocení laterální lumbální intervertebrální dézy hybridním biodegradabilním nanokompozitním porézním implantátem.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

1801-7576

Svazek periodika

90

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

69-75

Kód UT WoS článku

000654628800009

EID výsledku v databázi Scopus

2-s2.0-85104823198