The Micro-CT Analysis of the Structural Parameters of Collagen-based Porous Scaffolds: The Influence of Image Processing and Binarization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985891%3A_____%2F23%3A00566717" target="_blank" >RIV/67985891:_____/23:00566717 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11230/23:10453464 RIV/00216208:11110/23:10453464 RIV/00064165:_____/23:10453464

Výsledek na webu

<a href="https://doi.org/10.1093/micmic/ozac024" target="_blank" >https://doi.org/10.1093/micmic/ozac024</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/micmic/ozac024" target="_blank" >10.1093/micmic/ozac024</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Micro-CT Analysis of the Structural Parameters of Collagen-based Porous Scaffolds: The Influence of Image Processing and Binarization

Popis výsledku v původním jazyce

The micro-CT evaluation of structural parameter values is dependent on the processing of the image prior to its analysis. However, the degree of bias introduced by the image-processing process is generally unknown. The micro-CT analysis of three basic parameters (volume, surface, and pore size) was performed for 20 collagen-based porous scaffolds. The image data were processed using various approaches (noise reduction, binarization), analyzed and results were compared. A high degree of variability was observed in the values of the structural parameters. The differences between the mean values were as follows: 190% (volume), 160% (surface density), and 210% (pore size). Significant differences were observed with respect to the noise reduction in terms of the pore size values (frequently in excess of 300%). Our study proved the significant dependence of image processing on the results obtained. The data presented can be used as an estimate for the introduced bias. We suggest improvements in terms of the selection of the image-processing approach based on a combination of subjective selection (the standard method) and numerical analysis based on the consistency of the results. Although we focused on tissue engineering scaffolds, the principles are similar for all fields of the micro-CT application.

Název v anglickém jazyce

The Micro-CT Analysis of the Structural Parameters of Collagen-based Porous Scaffolds: The Influence of Image Processing and Binarization

Popis výsledku anglicky

The micro-CT evaluation of structural parameter values is dependent on the processing of the image prior to its analysis. However, the degree of bias introduced by the image-processing process is generally unknown. The micro-CT analysis of three basic parameters (volume, surface, and pore size) was performed for 20 collagen-based porous scaffolds. The image data were processed using various approaches (noise reduction, binarization), analyzed and results were compared. A high degree of variability was observed in the values of the structural parameters. The differences between the mean values were as follows: 190% (volume), 160% (surface density), and 210% (pore size). Significant differences were observed with respect to the noise reduction in terms of the pore size values (frequently in excess of 300%). Our study proved the significant dependence of image processing on the results obtained. The data presented can be used as an estimate for the introduced bias. We suggest improvements in terms of the selection of the image-processing approach based on a combination of subjective selection (the standard method) and numerical analysis based on the consistency of the results. Although we focused on tissue engineering scaffolds, the principles are similar for all fields of the micro-CT application.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30106 - Anatomy and morphology (plant science to be 1.6)

Projekt

<a href="/cs/project/GA20-11186S" target="_blank" >GA20-11186S: Mechanika tepenné delaminace a šíření trhliny</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy and Microanalysis

ISSN

1431-9276

e-ISSN

1435-8115

Svazek periodika

29

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

244-253

Kód UT WoS článku

001033590800024

EID výsledku v databázi Scopus

2-s2.0-85153395811