Vliv vybraných endokrinních disruptorů na meiotické zrání, expanzi kumulu, syntézu hyaluronové kyseliny a progesteronu oocyt-kumulárními komplexy prasat.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F09%3A00323300" target="_blank" >RIV/67985904:_____/09:00323300 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effects of selected endocrine disruptors on meiotic maturation, cumulus expansion, syntesis of hyaluronan and progesterone by porcine oocyte-cumulus complexes

Popis výsledku v původním jazyce

Before ovulation, cumulus cells synthesize a large amount of hyaluronan (HA) that is organized in an extracellular matrix.This process is called cumulus expansion. We assessed effects of endocrine disruptors (bisphenol A, BPA, 4-chloro-3-methyl phenol, CMP, di(2-ethylhexyl) phthalate, DEHP, and benzyl butyl phthalate, BBP) on FSH-induced meiotic maturation of porcine oocyte-cumulus complexes (OCC) cultured in vitro. BPA and CMP, significantly affected meiotic maturation of OCC. The number of oocytes that underwent GVBD; 78.7% and 72.4% as well as the rate of oocytes that reached M II; 50% and 53.6%, were decreased compared to control: GVBD-89.6% ; M II- 81.5%. FSH-stimulated expansion of cumulus was altered by the highest concentration of BPA and CMP (70% and 64% vs. 80.3% in control). Although BPA did not alter FSH-stimulated HA synthesis by cumulus cells, its incorporation within the complex was reduced to a half of control value. Progesterone production by OCC was significantly chan

Název v anglickém jazyce

Effects of selected endocrine disruptors on meiotic maturation, cumulus expansion, syntesis of hyaluronan and progesterone by porcine oocyte-cumulus complexes

Popis výsledku anglicky

Before ovulation, cumulus cells synthesize a large amount of hyaluronan (HA) that is organized in an extracellular matrix.This process is called cumulus expansion. We assessed effects of endocrine disruptors (bisphenol A, BPA, 4-chloro-3-methyl phenol, CMP, di(2-ethylhexyl) phthalate, DEHP, and benzyl butyl phthalate, BBP) on FSH-induced meiotic maturation of porcine oocyte-cumulus complexes (OCC) cultured in vitro. BPA and CMP, significantly affected meiotic maturation of OCC. The number of oocytes that underwent GVBD; 78.7% and 72.4% as well as the rate of oocytes that reached M II; 50% and 53.6%, were decreased compared to control: GVBD-89.6% ; M II- 81.5%. FSH-stimulated expansion of cumulus was altered by the highest concentration of BPA and CMP (70% and 64% vs. 80.3% in control). Although BPA did not alter FSH-stimulated HA synthesis by cumulus cells, its incorporation within the complex was reduced to a half of control value. Progesterone production by OCC was significantly chan

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA305%2F05%2F0960" target="_blank" >GA305/05/0960: Imunodetekce strukturalních komponent kumulu-oophoru, nezbytných pro úspěšné oplození.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicology in Vitro

ISSN

0887-2333

e-ISSN

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Svazek periodika

23

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000264510200002

EID výsledku v databázi Scopus

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