Activity of phytate dephosphorylation in cereals, legumes and oilseeds determined by colourimetric and isotachophoretic method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F11%3A00368275" target="_blank" >RIV/67985904:_____/11:00368275 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027014:_____/11:#0001457

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Activity of phytate dephosphorylation in cereals, legumes and oilseeds determined by colourimetric and isotachophoretic method

Popis výsledku v původním jazyce

Activity of phytate dephosphorylation was determined in 26 samples of cereals, legumes, oilseeds and complete diets for poultry using the colourimetric and isotachophoretic method. The former method was based on the colour formed upon the reaction of molybdate with phosphate released from phytate. The latter method was based on measurement of decrease of phytate concentration in the reaction mixture. Rye had by far the highest phytate dephosphorylation activity, followed by wheat and wheat-containing diets. However, for most of the other samples, this activity was low. Phytate dephosphorylation activity as measured by capillary isotachophoresis was numerically lower than activity measured using the molybdate method; however, activities assayed by bothmethods correlated with statistical significance (r=0.86; P<0.001). The isotachophoretic method may be suitable for phytate dephosphorylation activity assay in feeds with a high content of inorganic phosphate and in samples of digesta and

Název v anglickém jazyce

Activity of phytate dephosphorylation in cereals, legumes and oilseeds determined by colourimetric and isotachophoretic method

Popis výsledku anglicky

Activity of phytate dephosphorylation was determined in 26 samples of cereals, legumes, oilseeds and complete diets for poultry using the colourimetric and isotachophoretic method. The former method was based on the colour formed upon the reaction of molybdate with phosphate released from phytate. The latter method was based on measurement of decrease of phytate concentration in the reaction mixture. Rye had by far the highest phytate dephosphorylation activity, followed by wheat and wheat-containing diets. However, for most of the other samples, this activity was low. Phytate dephosphorylation activity as measured by capillary isotachophoresis was numerically lower than activity measured using the molybdate method; however, activities assayed by bothmethods correlated with statistical significance (r=0.86; P<0.001). The isotachophoretic method may be suitable for phytate dephosphorylation activity assay in feeds with a high content of inorganic phosphate and in samples of digesta and

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/GD525%2F08%2FH060" target="_blank" >GD525/08/H060: Využití synbiotik a rostlinných extraktů ve výživě lidí a zvířat</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Animal and Feed Sciences

ISSN

1230-1388

e-ISSN

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Svazek periodika

20

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

8

Strana od-do

472-479

Kód UT WoS článku

000294933400013

EID výsledku v databázi Scopus

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