The embryonic nucleologenesis during inhibition of major transcriptional activity in bovine preimplantation embryos
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F12%3A00390776" target="_blank" >RIV/67985904:_____/12:00390776 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.2478/s11756-012-0066-1" target="_blank" >http://dx.doi.org/10.2478/s11756-012-0066-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/s11756-012-0066-1" target="_blank" >10.2478/s11756-012-0066-1</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The embryonic nucleologenesis during inhibition of major transcriptional activity in bovine preimplantation embryos
Popis výsledku v původním jazyce
Common features of embryonic genome activation in mammalian and non-mammalian embryos are the colocalization of pre-assembled complexes of maternally inherited nucleolar proteins, the so-called nucleolus precursor bodies and de novo synthesized transcripts with ribosomal DNA. The de novo transcription of messenger RNA and ribosomal RNA proteins is required for the development of functional nuclei during the major activation of the embryonic genome. The aim of our work was to investigate to what extent.Autoradiography and transmission electron microscopy has been applied in in vitro produced bovine embryos. The embryos were cultured to the late 8-cell stage with: alpha-amanitin; a specific inhibitor of RNA-polymerases II and III transcription; actinomycin D; a specific inhibitor of RNA polymerase I transcription; and without inhibitors (control group). Nucleoplasm and nucleolar structures displayed strong autoradiographic labeling and showed the initial development of fibrillo-granular
Název v anglickém jazyce
The embryonic nucleologenesis during inhibition of major transcriptional activity in bovine preimplantation embryos
Popis výsledku anglicky
Common features of embryonic genome activation in mammalian and non-mammalian embryos are the colocalization of pre-assembled complexes of maternally inherited nucleolar proteins, the so-called nucleolus precursor bodies and de novo synthesized transcripts with ribosomal DNA. The de novo transcription of messenger RNA and ribosomal RNA proteins is required for the development of functional nuclei during the major activation of the embryonic genome. The aim of our work was to investigate to what extent.Autoradiography and transmission electron microscopy has been applied in in vitro produced bovine embryos. The embryos were cultured to the late 8-cell stage with: alpha-amanitin; a specific inhibitor of RNA-polymerases II and III transcription; actinomycin D; a specific inhibitor of RNA polymerase I transcription; and without inhibitors (control group). Nucleoplasm and nucleolar structures displayed strong autoradiographic labeling and showed the initial development of fibrillo-granular
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Biologia
ISSN
0006-3088
e-ISSN
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Svazek periodika
67
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
SK - Slovenská republika
Počet stran výsledku
8
Strana od-do
818-825
Kód UT WoS článku
000305840100026
EID výsledku v databázi Scopus
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