Reclassification of Eubacterium rectale (Hauduroy et al. 1937) Prevot 1938 in a new genus Agathobacter gen. nov as Agathobacter rectalis comb. nov., and description of Agathobacter ruminis sp nov., isolated from the rumen contents of sheep and cows

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F16%3A00458915" target="_blank" >RIV/67985904:_____/16:00458915 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/16:00458915

Výsledek na webu

<a href="http://dx.doi.org/10.1099/ijsem.0.000788" target="_blank" >http://dx.doi.org/10.1099/ijsem.0.000788</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1099/ijsem.0.000788" target="_blank" >10.1099/ijsem.0.000788</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Reclassification of Eubacterium rectale (Hauduroy et al. 1937) Prevot 1938 in a new genus Agathobacter gen. nov as Agathobacter rectalis comb. nov., and description of Agathobacter ruminis sp nov., isolated from the rumen contents of sheep and cows

Popis výsledku v původním jazyce

Three strains of a butyrate-producing bacterium were isolated from the rumen contents of grazing sheep and cows. The strains were anaerobic, with Gram-positive cell walls, straight-to-slightly-curved, rod-shaped, non-spore-forming and single flagellate. C-14 : 1, C-14 : (0), C-16 : 0 and C-16 : 1 were the predominant fatty acids. The cell-wall peptidoglycan type was A1 gamma. The DNA G+C content varied from 41.4 to 42.2 mol%. 16S rRNA gene sequence similarities between the isolates and Eubacterium rectale, Roseburia hominis and Roseburia intestinalis were found to be 96, 95 and 95 %, respectively. The phylogenetic tree showed that the strains constituted a different taxon, separate from other taxa with validly published names and forming a cluster with strains of Eubacterium rectale. On the basis of phenotypic, chemotaxonomic and phylogenetic results (16S RNA, dnaK, groEL, atpA genes), the isolates are considered to represent a novel species of a new genus of the family Lachnospiraceae, for which the name Agathobacter ruminis gen. nov., sp. nov. is proposed (type strain JK623(T) 5DSM 29029(T) 5LMG 28559(T)). We also propose the transfer of Eubacterium rectale to the new genus as Agathobacter rectalis gen. nov., comb nov. This new genus represents saccharoclastic, chemo-organotrophic and obligatory anaerobic, non-spore-forming rods with Gram-positive membrane. The main fermentation products on peptone yeast glucose (PYG) medium were butyrate, acetate, hydrogen and lactate. The type species of the genus is Agathobacter rectalis gen. nov., comb nov. (Prevot, 1938) with type strain ATCC 33656(T) (=JCM 17463(T)).

Název v anglickém jazyce

Reclassification of Eubacterium rectale (Hauduroy et al. 1937) Prevot 1938 in a new genus Agathobacter gen. nov as Agathobacter rectalis comb. nov., and description of Agathobacter ruminis sp nov., isolated from the rumen contents of sheep and cows

Popis výsledku anglicky

Three strains of a butyrate-producing bacterium were isolated from the rumen contents of grazing sheep and cows. The strains were anaerobic, with Gram-positive cell walls, straight-to-slightly-curved, rod-shaped, non-spore-forming and single flagellate. C-14 : 1, C-14 : (0), C-16 : 0 and C-16 : 1 were the predominant fatty acids. The cell-wall peptidoglycan type was A1 gamma. The DNA G+C content varied from 41.4 to 42.2 mol%. 16S rRNA gene sequence similarities between the isolates and Eubacterium rectale, Roseburia hominis and Roseburia intestinalis were found to be 96, 95 and 95 %, respectively. The phylogenetic tree showed that the strains constituted a different taxon, separate from other taxa with validly published names and forming a cluster with strains of Eubacterium rectale. On the basis of phenotypic, chemotaxonomic and phylogenetic results (16S RNA, dnaK, groEL, atpA genes), the isolates are considered to represent a novel species of a new genus of the family Lachnospiraceae, for which the name Agathobacter ruminis gen. nov., sp. nov. is proposed (type strain JK623(T) 5DSM 29029(T) 5LMG 28559(T)). We also propose the transfer of Eubacterium rectale to the new genus as Agathobacter rectalis gen. nov., comb nov. This new genus represents saccharoclastic, chemo-organotrophic and obligatory anaerobic, non-spore-forming rods with Gram-positive membrane. The main fermentation products on peptone yeast glucose (PYG) medium were butyrate, acetate, hydrogen and lactate. The type species of the genus is Agathobacter rectalis gen. nov., comb nov. (Prevot, 1938) with type strain ATCC 33656(T) (=JCM 17463(T)).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Systematic and Evolutionary Microbiology

ISSN

1466-5026

e-ISSN

—

Svazek periodika

66

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

768-773

Kód UT WoS článku

000372335600036

EID výsledku v databázi Scopus

2-s2.0-84960464428