Minute Y chromosomes and karyotype evolution in Madagascan iguanas (Squamata: Iguania: Opluridae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F16%3A00462603" target="_blank" >RIV/67985904:_____/16:00462603 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/16:10325420

Výsledek na webu

<a href="http://dx.doi.org/10.1111/bij.12751" target="_blank" >http://dx.doi.org/10.1111/bij.12751</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/bij.12751" target="_blank" >10.1111/bij.12751</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Minute Y chromosomes and karyotype evolution in Madagascan iguanas (Squamata: Iguania: Opluridae)

Popis výsledku v původním jazyce

Iguanas (Pleurodonta) are predominantly distributed in the New World, but one previously cytogenetically understudied family, Opluridae, is endemic to Madagascar and the adjacent Grand Comoro archipelago. The aim of our contribution is to fill a gap in the cytogenetic understanding of this biogeographically puzzling lineage. Based on examination of six species, we found that oplurids are rather conservative in karyotype, which is composed of 36 chromosomes as in most iguanas. However, the species differ in the position of the nucleolar organizer region and heterochromatic blocks and in the accumulation and distribution of interstitial telomeric sequences (ITSs), which suggests cryptic intra-and interchromosomal rearrangements. All tested species share the XY sex-determining system homologous to most other iguana families. The oplurid Y chromosome is degenerated, very small in size but mostly euchromatic. Fluorescence in situ hybridization with probes composed of microsatellite motifs revealed variability among species in the accumulation of particular repeats on the Y chromosome. This variability accounts for the differences in the detection of sex chromosomes across the species of the family using comparative genome hybridization (CGH) technique. Our study demonstrates the limits of the commonly used CGH technique to uncover sex chromosomes even in organisms with heteromorphic and sequentially largely differentiated sex chromosomes.

Název v anglickém jazyce

Minute Y chromosomes and karyotype evolution in Madagascan iguanas (Squamata: Iguania: Opluridae)

Popis výsledku anglicky

Iguanas (Pleurodonta) are predominantly distributed in the New World, but one previously cytogenetically understudied family, Opluridae, is endemic to Madagascar and the adjacent Grand Comoro archipelago. The aim of our contribution is to fill a gap in the cytogenetic understanding of this biogeographically puzzling lineage. Based on examination of six species, we found that oplurids are rather conservative in karyotype, which is composed of 36 chromosomes as in most iguanas. However, the species differ in the position of the nucleolar organizer region and heterochromatic blocks and in the accumulation and distribution of interstitial telomeric sequences (ITSs), which suggests cryptic intra-and interchromosomal rearrangements. All tested species share the XY sex-determining system homologous to most other iguana families. The oplurid Y chromosome is degenerated, very small in size but mostly euchromatic. Fluorescence in situ hybridization with probes composed of microsatellite motifs revealed variability among species in the accumulation of particular repeats on the Y chromosome. This variability accounts for the differences in the detection of sex chromosomes across the species of the family using comparative genome hybridization (CGH) technique. Our study demonstrates the limits of the commonly used CGH technique to uncover sex chromosomes even in organisms with heteromorphic and sequentially largely differentiated sex chromosomes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EG - Zoologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP506%2F10%2F0718" target="_blank" >GAP506/10/0718: Evoluce pohlavně determinačních mechanismů šupinatých plazů: molekulárně cytogenetický test nezávislého vzniku pohlavních chromozomů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biological Journal of the Linnean Society

ISSN

0024-4066

e-ISSN

—

Svazek periodika

118

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

16

Strana od-do

618-633

Kód UT WoS článku

000379783400014

EID výsledku v databázi Scopus

2-s2.0-84957837556