Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F16%3A00465124" target="_blank" >RIV/67985904:_____/16:00465124 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.livsci.2016.08.001" target="_blank" >http://dx.doi.org/10.1016/j.livsci.2016.08.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.livsci.2016.08.001" target="_blank" >10.1016/j.livsci.2016.08.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage

Popis výsledku v původním jazyce

Using culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bloat. Changes of the bacterial composition of rumen were monitored using DGGE analysis of 16S rDNA gene amplicons and quantitative PCR method. As the diet changed, the bacterial population of Bacteroidetes and gamma-Proteobacteria decreased, even if animals have not shown signs of frothy bloat. The most severely bloated animals showed an increase of Bacteroidetes phylum. Lactobacillus/Streptococcus group belonging to Firmicutes phylum decreased in response to transferring the animals from hay to a fresh forage-based diet, and did not achieve the values observed at the beginning of the experiment. In summary, changes in the diet and subsequent frothy bloat occurrence produce long-lasting changes in the structure of the microbial community and may be associated with a specific bacterial population belonging to the Bacteroidetes phylum.

Název v anglickém jazyce

Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage

Popis výsledku anglicky

Using culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bloat. Changes of the bacterial composition of rumen were monitored using DGGE analysis of 16S rDNA gene amplicons and quantitative PCR method. As the diet changed, the bacterial population of Bacteroidetes and gamma-Proteobacteria decreased, even if animals have not shown signs of frothy bloat. The most severely bloated animals showed an increase of Bacteroidetes phylum. Lactobacillus/Streptococcus group belonging to Firmicutes phylum decreased in response to transferring the animals from hay to a fresh forage-based diet, and did not achieve the values observed at the beginning of the experiment. In summary, changes in the diet and subsequent frothy bloat occurrence produce long-lasting changes in the structure of the microbial community and may be associated with a specific bacterial population belonging to the Bacteroidetes phylum.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Livestock Science

ISSN

1871-1413

e-ISSN

—

Svazek periodika

191

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

191-195

Kód UT WoS článku

000383314600030

EID výsledku v databázi Scopus

2-s2.0-84982085683