Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F17%3A00472227" target="_blank" >RIV/67985904:_____/17:00472227 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21230/17:00307540 RIV/68407700:21730/17:00307540 RIV/00216224:14310/17:00096114 RIV/62156489:43210/17:43910835 RIV/00216305:26620/17:PU122784

Výsledek na webu

<a href="http://dx.doi.org/10.1038/srep40169" target="_blank" >http://dx.doi.org/10.1038/srep40169</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/srep40169" target="_blank" >10.1038/srep40169</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration

Popis výsledku v původním jazyce

We describe a new procedure for the parallel mapping of selected metals in histologically characterized tissue samples. Mapping is achieved via image registration of digital data obtained from two neighbouring cryosections by scanning the first as a histological sample and subjecting the second to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure enables determination of the distribution and content of metals of interest directly in the chosen histological zones and represents a substantial improvement over the standard approach, which determines these values in tissue homogenates or whole tissue sections. The potential of the described procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We anticipate that the procedure could be useful for a complex understanding of the role that the spatial distribution of metals plays within tissues affected by pathological states including cancer.

Název v anglickém jazyce

Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration

Popis výsledku anglicky

We describe a new procedure for the parallel mapping of selected metals in histologically characterized tissue samples. Mapping is achieved via image registration of digital data obtained from two neighbouring cryosections by scanning the first as a histological sample and subjecting the second to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure enables determination of the distribution and content of metals of interest directly in the chosen histological zones and represents a substantial improvement over the standard approach, which determines these values in tissue homogenates or whole tissue sections. The potential of the described procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We anticipate that the procedure could be useful for a complex understanding of the role that the spatial distribution of metals plays within tissues affected by pathological states including cancer.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

—

Kód UT WoS článku

000392234200001

EID výsledku v databázi Scopus

2-s2.0-85009230317