Prostaglandin E2 stimulates the expression of cumulus expansion-related genes in pigs: the role of protein kinase B

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F17%3A00475936" target="_blank" >RIV/67985904:_____/17:00475936 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/17:10338406

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.prostaglandins.2017.04.001" target="_blank" >http://dx.doi.org/10.1016/j.prostaglandins.2017.04.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.prostaglandins.2017.04.001" target="_blank" >10.1016/j.prostaglandins.2017.04.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Prostaglandin E2 stimulates the expression of cumulus expansion-related genes in pigs: the role of protein kinase B

Popis výsledku v původním jazyce

The production of prostaglandin E2 (PGE2) seems to play an important role in the ovulation process. PGE2 was found to induce cumulus expansion and meiosis resumption in mice, but little is known about its role in pigs. The goals of this study were (a) to assess the effect of PGE2 on the expression levels of cumulus expansion-related genes, (b) to define the signaling pathways that drive the PGE2-stimulated expression of cumulus expansion related genes, (c) to measure the effect of PGE2 on the activation of key signaling molecules (MAPK3/1, PKB) and on hyaluronan production in cumulus cells, and (d) to assess the effect of PGE2 on meiosis resumption. We documented that PGE2 is able to induce the expression of cumulus expansion-related genes (HAS2, TNFAIP6) as well as genes involved in steroidogenesis (CYP11A1) or prostaglandin production (PTGS2). PGE2 is able to activate PKB and MAPK3/1 and induce mild cumulus expansion and meiosis resumption, but less efficiently than FSH.

Název v anglickém jazyce

Prostaglandin E2 stimulates the expression of cumulus expansion-related genes in pigs: the role of protein kinase B

Popis výsledku anglicky

The production of prostaglandin E2 (PGE2) seems to play an important role in the ovulation process. PGE2 was found to induce cumulus expansion and meiosis resumption in mice, but little is known about its role in pigs. The goals of this study were (a) to assess the effect of PGE2 on the expression levels of cumulus expansion-related genes, (b) to define the signaling pathways that drive the PGE2-stimulated expression of cumulus expansion related genes, (c) to measure the effect of PGE2 on the activation of key signaling molecules (MAPK3/1, PKB) and on hyaluronan production in cumulus cells, and (d) to assess the effect of PGE2 on meiosis resumption. We documented that PGE2 is able to induce the expression of cumulus expansion-related genes (HAS2, TNFAIP6) as well as genes involved in steroidogenesis (CYP11A1) or prostaglandin production (PTGS2). PGE2 is able to activate PKB and MAPK3/1 and induce mild cumulus expansion and meiosis resumption, but less efficiently than FSH.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Prostaglandins & Other Lipid Mediators

ISSN

1098-8823

e-ISSN

—

Svazek periodika

130

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

38-46

Kód UT WoS článku

000403527200006

EID výsledku v databázi Scopus

2-s2.0-85017374167