Local application of adipose-derived mesenchymal stem cells supports the healing of fistula: prospective randomised study on rat model of fistulising Crohn's disease

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F17%3A00475938" target="_blank" >RIV/67985904:_____/17:00475938 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023001:_____/17:00060294 RIV/00064211:_____/17:W0000044 RIV/00216208:11120/17:43912902

Výsledek na webu

<a href="http://dx.doi.org/10.1080/00365521.2017.1281434" target="_blank" >http://dx.doi.org/10.1080/00365521.2017.1281434</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/00365521.2017.1281434" target="_blank" >10.1080/00365521.2017.1281434</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Local application of adipose-derived mesenchymal stem cells supports the healing of fistula: prospective randomised study on rat model of fistulising Crohn's disease

Popis výsledku v původním jazyce

Objective: Local application of adipose-derived mesenchymal stem cells (ADSC) represents a novel approach for the management of perianal fistula in patients with Crohn's disease. A randomised study on an animal model was performed to investigate the efficacy and to detect the distribution of implanted ADSCs by bioluminescence (BLI). nMaterials and methods: A caecostomy was used as a fistula model in 32 Lewis rats. The ADSCs were isolated from transgenic donor expressing firefly luciferase. Animals were randomly assigned to groups given injections of 4x10(6) cells (n=16, group A) or placebo (n=16, group B) in the perifistular tissue. Fistula drainage assessment was used to evaluate the fistula healing. After application of D-luciferin, cell viability and distribution was detected using an IVIS Lumina XR camera on days 0, 2, 7, 14 and 30. nResults: The fistula was identified as healed in 6 (38%) animals in group A vs. 1 case (6.3%) in group B (p=.033). The BLI was strongest immediately after administration of ADSCs 31.2x10(4) (6.09-111x10(4)) p/s/cm(2)/sr. The fastest decrease was observed within the first 2 days when values fell by 50.2%. The BLI 30 days after injection was significantly higher in animals with healed fistulas - 8.23x10(4) (1.18-16.9x10(4)) vs. 1.74x10(4) (0.156-6.88x10(4)), p=.0393. nConclusions: Local application of ADSCs resulted in significantly higher fistula closure rate on an animal model. BLI monitoring was proved to be feasible and showed rapid reduction of the ADSC mass after application. More viable cells were detected in animals with healed fistula at the end of the follow-up.

Název v anglickém jazyce

Local application of adipose-derived mesenchymal stem cells supports the healing of fistula: prospective randomised study on rat model of fistulising Crohn's disease

Popis výsledku anglicky

Objective: Local application of adipose-derived mesenchymal stem cells (ADSC) represents a novel approach for the management of perianal fistula in patients with Crohn's disease. A randomised study on an animal model was performed to investigate the efficacy and to detect the distribution of implanted ADSCs by bioluminescence (BLI). nMaterials and methods: A caecostomy was used as a fistula model in 32 Lewis rats. The ADSCs were isolated from transgenic donor expressing firefly luciferase. Animals were randomly assigned to groups given injections of 4x10(6) cells (n=16, group A) or placebo (n=16, group B) in the perifistular tissue. Fistula drainage assessment was used to evaluate the fistula healing. After application of D-luciferin, cell viability and distribution was detected using an IVIS Lumina XR camera on days 0, 2, 7, 14 and 30. nResults: The fistula was identified as healed in 6 (38%) animals in group A vs. 1 case (6.3%) in group B (p=.033). The BLI was strongest immediately after administration of ADSCs 31.2x10(4) (6.09-111x10(4)) p/s/cm(2)/sr. The fastest decrease was observed within the first 2 days when values fell by 50.2%. The BLI 30 days after injection was significantly higher in animals with healed fistulas - 8.23x10(4) (1.18-16.9x10(4)) vs. 1.74x10(4) (0.156-6.88x10(4)), p=.0393. nConclusions: Local application of ADSCs resulted in significantly higher fistula closure rate on an animal model. BLI monitoring was proved to be feasible and showed rapid reduction of the ADSC mass after application. More viable cells were detected in animals with healed fistula at the end of the follow-up.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30219 - Gastroenterology and hepatology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scandinavian Journal of Gastroenterology

ISSN

0036-5521

e-ISSN

—

Svazek periodika

52

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

NO - Norské království

Počet stran výsledku

8

Strana od-do

543-550

Kód UT WoS článku

000395746800008

EID výsledku v databázi Scopus

2-s2.0-85010653669