Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F17%3A00479032" target="_blank" >RIV/67985904:_____/17:00479032 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081766:_____/17:00478269 RIV/00216224:14310/17:00100372 RIV/00216208:11310/17:10369393

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ygcen.2017.06.023" target="_blank" >http://dx.doi.org/10.1016/j.ygcen.2017.06.023</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ygcen.2017.06.023" target="_blank" >10.1016/j.ygcen.2017.06.023</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus)

Popis výsledku v původním jazyce

Long term changes in hormonal levels of small and rare animal species can be effectively monitored by non-invasive methods such as immunoenzymatic analysis. These methods generally analyze metabolites instead of the hormone itself and thus do not invade the organism. However, they can be influenced by many factors, so before they are used, they need to be validated. For this purpose we used the ACM challenge test based on stimulating the animal's adrenocortical activity and measuring the consequent increase of the level of its glucocorticoid (stress) hormones. Specifically, we measured concentrations of fecal glucocorticoid metabolites of two house mouse subspecies, Mus musculus musculus and M. m. domesticus. Using polyclonal antibody we investigated the mice's naturally occurring circadian fluctuation and their reaction to the adrenal stimulation. This study confirmed that the selected method is suitable for analysis of fecal corticosterone metabolites in the wild house mouse. More importantly, we revealed a subspecies-specific stress response at the level of corticosterone production: while the significant effect of ACTH was proved in both subspecies, a notable adrenocortical reaction was also elicited by injecting the saline solution in M. m. domesticus. Our results thus highlight the importance of considering potential cryptic variation within the species under study when designing projects on adrenal stress hormone assessments.

Název v anglickém jazyce

Subspecies-specific response to ACTH challenge test in the house mouse (Mus musculus)

Popis výsledku anglicky

Long term changes in hormonal levels of small and rare animal species can be effectively monitored by non-invasive methods such as immunoenzymatic analysis. These methods generally analyze metabolites instead of the hormone itself and thus do not invade the organism. However, they can be influenced by many factors, so before they are used, they need to be validated. For this purpose we used the ACM challenge test based on stimulating the animal's adrenocortical activity and measuring the consequent increase of the level of its glucocorticoid (stress) hormones. Specifically, we measured concentrations of fecal glucocorticoid metabolites of two house mouse subspecies, Mus musculus musculus and M. m. domesticus. Using polyclonal antibody we investigated the mice's naturally occurring circadian fluctuation and their reaction to the adrenal stimulation. This study confirmed that the selected method is suitable for analysis of fecal corticosterone metabolites in the wild house mouse. More importantly, we revealed a subspecies-specific stress response at the level of corticosterone production: while the significant effect of ACTH was proved in both subspecies, a notable adrenocortical reaction was also elicited by injecting the saline solution in M. m. domesticus. Our results thus highlight the importance of considering potential cryptic variation within the species under study when designing projects on adrenal stress hormone assessments.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Projekt

<a href="/cs/project/GAP506%2F11%2F1792" target="_blank" >GAP506/11/1792: Populační struktura, disperze a explorační chování v zóně sekundárního kontaktu myší domácích</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

General and Comparative Endocrinology

ISSN

0016-6480

e-ISSN

—

Svazek periodika

252

Číslo periodika v rámci svazku

October

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

186-192

Kód UT WoS článku

000409396400019

EID výsledku v databázi Scopus

2-s2.0-85024472239