Periderm invasion contributes to epithelial formation in the teleost pharynx

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F19%3A00517157" target="_blank" >RIV/67985904:_____/19:00517157 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-019-46040-y" target="_blank" >https://www.nature.com/articles/s41598-019-46040-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-019-46040-y" target="_blank" >10.1038/s41598-019-46040-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Periderm invasion contributes to epithelial formation in the teleost pharynx

Popis výsledku v původním jazyce

The gnathostome pharyngeal cavity functions in food transport and respiration. In amniotes the mouth and nares are the only channels allowing direct contact between internal and external epithelia. In teleost fish, gill slits arise through opening of endodermal pouches and connect the pharynx to the exterior. Using transgenic zebrafish lines, cell tracing, live imaging and different markers, we investigated if pharyngeal openings enable epithelial invasion and how this modifies the pharyngeal epithelium. We conclude that in zebrafish the pharyngeal endoderm becomes overlain by cells with a peridermal phenotype. In a wave starting from pouch 2, peridermal cells from the outer skin layer invade the successive pouches until halfway their depth. Here the peridermal cells connect to a population of cells inside the pharyngeal cavity that express periderm markers, yet do not invade from outside. The latter population expands along the midline from anterior to posterior until the esophagusgut boundary. Together, our results show a novel role for the periderm as an internal epithelium becomes adapted to function as an external surface.

Název v anglickém jazyce

Periderm invasion contributes to epithelial formation in the teleost pharynx

Popis výsledku anglicky

The gnathostome pharyngeal cavity functions in food transport and respiration. In amniotes the mouth and nares are the only channels allowing direct contact between internal and external epithelia. In teleost fish, gill slits arise through opening of endodermal pouches and connect the pharynx to the exterior. Using transgenic zebrafish lines, cell tracing, live imaging and different markers, we investigated if pharyngeal openings enable epithelial invasion and how this modifies the pharyngeal epithelium. We conclude that in zebrafish the pharyngeal endoderm becomes overlain by cells with a peridermal phenotype. In a wave starting from pouch 2, peridermal cells from the outer skin layer invade the successive pouches until halfway their depth. Here the peridermal cells connect to a population of cells inside the pharyngeal cavity that express periderm markers, yet do not invade from outside. The latter population expands along the midline from anterior to posterior until the esophagusgut boundary. Together, our results show a novel role for the periderm as an internal epithelium becomes adapted to function as an external surface.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cells

ISSN

2073-4409

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

JUL 12

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

10082

Kód UT WoS článku

000475292700004

EID výsledku v databázi Scopus

2-s2.0-85068970089