Inhibition of miR-152 during In Vitro Maturation Enhances the Developmental Potential of Porcine Embryos

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F20%3A00537771" target="_blank" >RIV/67985904:_____/20:00537771 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2076-2615/10/12/2289" target="_blank" >https://www.mdpi.com/2076-2615/10/12/2289</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ani10122289" target="_blank" >10.3390/ani10122289</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Inhibition of miR-152 during In Vitro Maturation Enhances the Developmental Potential of Porcine Embryos

Popis výsledku v původním jazyce

MiR-152 is a highly conserved miRNA across different species and plays a role in the regulation of cell differentiation, proliferation, and survival. However, the exact role of miR-152 in oocyte and embryo development is not yet known. In this study, we specifically manipulated the expression level of miR-152 in porcine cumulus-oocyte complexes (COCs) and monitored their developmental competence until the blastocyst stage. We mainly found that a suppressed expression of miR-152 during oocyte maturation significantly improved the blastocyst rate. Our results indicate that this negative correlation between miR-152 during oocyte maturation and the blastocyst rate in pigs could be through targeting IGF system components during oocyte development. These results provide more insights into the role of miRNAs during oocyte and embryonic development that could improve the in vitro production system for mammalian embryos.

Název v anglickém jazyce

Inhibition of miR-152 during In Vitro Maturation Enhances the Developmental Potential of Porcine Embryos

Popis výsledku anglicky

MiR-152 is a highly conserved miRNA across different species and plays a role in the regulation of cell differentiation, proliferation, and survival. However, the exact role of miR-152 in oocyte and embryo development is not yet known. In this study, we specifically manipulated the expression level of miR-152 in porcine cumulus-oocyte complexes (COCs) and monitored their developmental competence until the blastocyst stage. We mainly found that a suppressed expression of miR-152 during oocyte maturation significantly improved the blastocyst rate. Our results indicate that this negative correlation between miR-152 during oocyte maturation and the blastocyst rate in pigs could be through targeting IGF system components during oocyte development. These results provide more insights into the role of miRNAs during oocyte and embryonic development that could improve the in vitro production system for mammalian embryos.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

<a href="/cs/project/EF15_003%2F0000460" target="_blank" >EF15_003/0000460: EXCELENCE molekulárních aspektů časného vývoje obratlovců</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animals

ISSN

2076-2615

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

2289

Kód UT WoS článku

000601640800001

EID výsledku v databázi Scopus

2-s2.0-85097317672