Characterization of porcine oocytes stained with Lissamine Green B and their developmental potential in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F20%3A00550729" target="_blank" >RIV/67985904:_____/20:00550729 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.scielo.br/j/ar/a/7ywdBxWN8pbPVk4FC8WgtrJ/?lang=en" target="_blank" >https://www.scielo.br/j/ar/a/7ywdBxWN8pbPVk4FC8WgtrJ/?lang=en</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1590/1984-3143-AR2020-0533" target="_blank" >10.1590/1984-3143-AR2020-0533</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of porcine oocytes stained with Lissamine Green B and their developmental potential in vitro

Popis výsledku v původním jazyce

Traditional methods for the evaluation of oocyte quality are based on morphological classification of the follicle, cumulus-oocyte complex, polar body and meiotic spindle. This study is focused on the differences between the morphological assessment of oocyte quality, the assessment based on Lissamine Green B (LB) staining and the analysis of oocytes using a proteomic approach. We evaluated the effectiveness of electrochemical and chemical parthenogenetic activation under our laboratory conditions and evaluated the applicability of Lissamine Green B staining of cumulus-oocyte complexes (COCs) as a non-invasive method for predicting the maturational and developmental competence of porcine oocytes cultured in vitro. We determined that chemical parthenogenetic activation using ionomycin and 6-dimethylaminopurine was slightly more effective than electrochemical activation. After oocyte selection according to LB staining, we found significant differences (P<0.05) between the LB-group and LB+ group and the control group in their maturation, cleavage rate and rate of blastocysts. Proteomic analyses identified a selection of proteins that were differentially expressed in each group of analysed oocytes. Oocytes of the LB-group exhibited an increased variability of proteins involved in transcription regulation, proteosynthesis and the protein folding crucial for oocyte maturation and further embryonic development. These results found a better competence of LB-oocytes in maturation, cleavage and ability to reach the blastocyst stage.

Název v anglickém jazyce

Characterization of porcine oocytes stained with Lissamine Green B and their developmental potential in vitro

Popis výsledku anglicky

Traditional methods for the evaluation of oocyte quality are based on morphological classification of the follicle, cumulus-oocyte complex, polar body and meiotic spindle. This study is focused on the differences between the morphological assessment of oocyte quality, the assessment based on Lissamine Green B (LB) staining and the analysis of oocytes using a proteomic approach. We evaluated the effectiveness of electrochemical and chemical parthenogenetic activation under our laboratory conditions and evaluated the applicability of Lissamine Green B staining of cumulus-oocyte complexes (COCs) as a non-invasive method for predicting the maturational and developmental competence of porcine oocytes cultured in vitro. We determined that chemical parthenogenetic activation using ionomycin and 6-dimethylaminopurine was slightly more effective than electrochemical activation. After oocyte selection according to LB staining, we found significant differences (P<0.05) between the LB-group and LB+ group and the control group in their maturation, cleavage rate and rate of blastocysts. Proteomic analyses identified a selection of proteins that were differentially expressed in each group of analysed oocytes. Oocytes of the LB-group exhibited an increased variability of proteins involved in transcription regulation, proteosynthesis and the protein folding crucial for oocyte maturation and further embryonic development. These results found a better competence of LB-oocytes in maturation, cleavage and ability to reach the blastocyst stage.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

<a href="/cs/project/EF15_003%2F0000460" target="_blank" >EF15_003/0000460: EXCELENCE molekulárních aspektů časného vývoje obratlovců</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal Reproduction

ISSN

1806-9614

e-ISSN

1984-3143

Svazek periodika

17

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

BR - Brazilská federativní republika

Počet stran výsledku

11

Strana od-do

e20200533

Kód UT WoS článku

000645124300010

EID výsledku v databázi Scopus

2-s2.0-85098937476