Divergent gut microbiota in two closely related house mouse subspecies under common garden conditions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00561123" target="_blank" >RIV/67985904:_____/22:00561123 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081766:_____/22:00560422 RIV/00216208:11310/22:10447189

Výsledek na webu

<a href="https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=90812963689" target="_blank" >https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=90812963689</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femsec/fiac078" target="_blank" >10.1093/femsec/fiac078</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Divergent gut microbiota in two closely related house mouse subspecies under common garden conditions

Popis výsledku v původním jazyce

The gastrointestinal microbiota (GM) is considered an important component of the vertebrate holobiont. GM-host interactions influence the fitness of holobionts and are, therefore, an integral part of evolution. The house mouse is a prominent model for GM-host interactions, and evidence suggests a role for GM in mouse speciation. However, previous studies based on short 16S rRNA GM profiles of wild house mouse subspecies failed to detect GM divergence, which is a prerequisite for the inclusion of GM in Dobzhansky-Muller incompatibilities. Here, we used standard 16S rRNA GM profiling in two mouse subspecies, Mus musculus musculus and M. m. domesticus, including the intestinal mucosa and content of three gut sections (ileum, caecum, and colon). We reduced environmental variability by sampling GM in the offspring of wild mice bred under seminatural conditions. Although the breeding conditions allowed a contact between the subspecies, we found a clear differentiation of GM between them, in all three gut sections. Differentiation was mainly driven by several Helicobacters and two H. ganmani variants showed a signal of codivergence with their hosts. Helicobacters represent promising candidates for studying GM-host coadaptations and the fitness effects of their interactions.nnSequencing of bacterial 16S rRNA gene reveales differences in gut microbiota between two house mouse subspecies (mainly formed by genus Helicobacter), their gut segments, and between gut mucosa and content.

Název v anglickém jazyce

Divergent gut microbiota in two closely related house mouse subspecies under common garden conditions

Popis výsledku anglicky

The gastrointestinal microbiota (GM) is considered an important component of the vertebrate holobiont. GM-host interactions influence the fitness of holobionts and are, therefore, an integral part of evolution. The house mouse is a prominent model for GM-host interactions, and evidence suggests a role for GM in mouse speciation. However, previous studies based on short 16S rRNA GM profiles of wild house mouse subspecies failed to detect GM divergence, which is a prerequisite for the inclusion of GM in Dobzhansky-Muller incompatibilities. Here, we used standard 16S rRNA GM profiling in two mouse subspecies, Mus musculus musculus and M. m. domesticus, including the intestinal mucosa and content of three gut sections (ileum, caecum, and colon). We reduced environmental variability by sampling GM in the offspring of wild mice bred under seminatural conditions. Although the breeding conditions allowed a contact between the subspecies, we found a clear differentiation of GM between them, in all three gut sections. Differentiation was mainly driven by several Helicobacters and two H. ganmani variants showed a signal of codivergence with their hosts. Helicobacters represent promising candidates for studying GM-host coadaptations and the fitness effects of their interactions.nnSequencing of bacterial 16S rRNA gene reveales differences in gut microbiota between two house mouse subspecies (mainly formed by genus Helicobacter), their gut segments, and between gut mucosa and content.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FEMS Microbiology Ecology

ISSN

0168-6496

e-ISSN

1574-6941

Svazek periodika

98

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

fiac078

Kód UT WoS článku

000840866400001

EID výsledku v databázi Scopus

2-s2.0-85136908934