High-resolution ribosome profiling reveals translational selectivity for transcripts in bovine preimplantation embryo development

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00569567" target="_blank" >RIV/67985904:_____/22:00569567 - isvavai.cz</a>

Výsledek na webu

<a href="https://journals.biologists.com/dev/article/149/21/dev200819/280468/High-resolution-ribosome-profiling-reveals" target="_blank" >https://journals.biologists.com/dev/article/149/21/dev200819/280468/High-resolution-ribosome-profiling-reveals</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/dev.200819" target="_blank" >10.1242/dev.200819</a>

Jazyk výsledku

angličtina

Název v původním jazyce

High-resolution ribosome profiling reveals translational selectivity for transcripts in bovine preimplantation embryo development

Popis výsledku v původním jazyce

High-resolution ribosome fractionation and low-input ribosome profiling of bovine oocytes and preimplantation embryos has enabled us to define the translational landscapes of early embryo development at an unprecedented level. We analyzed the transcriptome and the polysome-and non-polysome-bound RNA profiles of bovine oocytes (germinal vesicle and metaphase II stages) and early embryos at the two-cell, eight-cell, morula and blastocyst stages, and revealed four modes of translational selectivity: (1) selective translation of non-abundant mRNAs, (2) active, but modest translation of a selection of highly expressed mRNAs, (3) translationally suppressed abundant to moderately abundant mRNAs, and (4) mRNAs associated specifically with monosomes. A strong translational selection of low-abundance transcripts involved in metabolic pathways and lysosomes was found throughout bovine embryonic development. Notably, genes involved in mitochondrial function were prioritized for translation. We found that translation largely reflected transcription in oocytes and two-cell embryos, but observed a marked shift in the translational control in eight-cell embryos that was associated with the main phase of embryonic genome activation. Subsequently, transcription and translation become more synchronized in morulae and blastocysts. Taken together, these data reveal a unique spatiotemporal translational regulation that accompanies bovine preimplantation development.

Název v anglickém jazyce

High-resolution ribosome profiling reveals translational selectivity for transcripts in bovine preimplantation embryo development

Popis výsledku anglicky

High-resolution ribosome fractionation and low-input ribosome profiling of bovine oocytes and preimplantation embryos has enabled us to define the translational landscapes of early embryo development at an unprecedented level. We analyzed the transcriptome and the polysome-and non-polysome-bound RNA profiles of bovine oocytes (germinal vesicle and metaphase II stages) and early embryos at the two-cell, eight-cell, morula and blastocyst stages, and revealed four modes of translational selectivity: (1) selective translation of non-abundant mRNAs, (2) active, but modest translation of a selection of highly expressed mRNAs, (3) translationally suppressed abundant to moderately abundant mRNAs, and (4) mRNAs associated specifically with monosomes. A strong translational selection of low-abundance transcripts involved in metabolic pathways and lysosomes was found throughout bovine embryonic development. Notably, genes involved in mitochondrial function were prioritized for translation. We found that translation largely reflected transcription in oocytes and two-cell embryos, but observed a marked shift in the translational control in eight-cell embryos that was associated with the main phase of embryonic genome activation. Subsequently, transcription and translation become more synchronized in morulae and blastocysts. Taken together, these data reveal a unique spatiotemporal translational regulation that accompanies bovine preimplantation development.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

<a href="/cs/project/GA22-27301S" target="_blank" >GA22-27301S: Přechod z meiózy do mitózy - je započetí nového života in vitro rovnocenné in vivo vývoji?</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Development

ISSN

0950-1991

e-ISSN

1477-9129

Svazek periodika

149

Číslo periodika v rámci svazku

21

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

dev200819

Kód UT WoS článku

000903918600004

EID výsledku v databázi Scopus

2-s2.0-85141889757